Liverpool Skylilne

Natural Sciences and Psychology

Dr Andrias O'Reilly

Dr Andrias O'Reilly

Telephone: 0151 231 2330

Biography

I investigate the structure and function of ion channel membrane proteins and study how they interact with drugs, toxins and environmental pollutants. I am particularly interested in voltage-gated sodium channels and my research focuses on (i) characterising receptor sites on sodium channels that bind insecticidal compounds and (ii) understanding how mutations of sodium channels can produce human pain disorders. I also clone, express and purify small-peptide neurotoxins from scorpions, spiders and other venomous creatures, as these molecules represent excellent pharmacology probes and have enormous potential as lead compounds for novel therapeutics and insecticides.


EDUCATION

2001-2005: PhD Molecular Biophysics, University of London, UK
1998-1999: MSc Molecular Modelling and Bioinformatics, University of London, UK
1995-1998: BSc in Biochemistry, Queen's University of Belfast, Northern Ireland


CURRENT POST

2014-present: Lecturer/Senior Lecturer in Neurobiology, Liverpool John Moores University, UK


PREVIOUS POSTS

2011-2014: Marie Curie Research Fellow, Institut für Physiologie und Pathophysiologie, Friedrich-Alexander-Universität, Erlangen, Germany
2007-2011: Post-doctoral Researcher, Department of Crystallography, Birkbeck College, University of London, UK
2005-2007: Post-doctoral Researcher, Rothamsted Research, Harpenden, Hertfordshire, UK


PROFESSIONAL MEMBERSHIPS

Biophysical Society
British Neuroscience Association
International Society on Toxinology


TEACHING

Module leader: 3046FNDSCI Anatomy and Physiology
Module leader: 6027NATSCI Neurobiology

4004NATSCI Physiology
4007NATSCI Physiological Concepts
5013NATSCI Genes & Genomes
5019NATSCI Physiology of Life
5020NATSCI Comparative Animal Physiology
6000NATSCI Research Project
6020NATSCI Health & Disease
6024NATSCI Current Topics in Zoology
6025NATSCI Applications of Genetics in Health and Disease

Degrees

2005, Birkbeck College, University of London, United Kingdom, PhD
1999, Birkbeck College, University of London, United Kingdom, MSc
1998, Queen's University of Belfast, Northern Ireland, BSc

Publications

Highlighted publications

O'Reilly AO, Cole AR, Lopes JLS, Lampert A, Wallace BA. 2014. Chaperone-mediated native folding of a beta-scorpion toxin in the periplasm of Escherichia coli BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1840 :10-15 >DOI >Link

O'Reilly AO, Williamson MS, Gonzalez-Cabrera J, Turberg A, Field LM, Wallace BA, Davies TGE. 2014. Predictive 3D modelling of the interactions of pyrethroids with the voltage-gated sodium channels of ticks and mites PEST MANAGEMENT SCIENCE, 70 :369-377 >DOI >Link

O'Reilly AO, Eberhardt E, Weidner C, Alzheimer C, Wallace BA, Lampert A. 2012. Bisphenol A Binds to the Local Anesthetic Receptor Site to Block the Human Cardiac Sodium Channel PLOS ONE, 7 >DOI >Link

Lampert A, O'Reilly AO, Reeh P, Leffler A. 2010. Sodium channelopathies and pain PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 460 :249-263 >DOI >Link

O'Reilly AO, Khambay BPS, Williamson MS, Field LM, Wallace BA, Davies TGE. 2006. Modelling insecticide-binding sites in the voltage-gated sodium channel Biochemical Journal, 396 :255-263 >DOI

Journal Articles

Maile CA, Hingst JR, Mahalingan KK, O'Reilly AO, Cleasby ME, Mickelson JR, McCue ME, Anderson SM, Hurley TD, Wojtaszewski JF, Piercy RJ. 2016. A highly prevalent equine glycogen storage disease is explained by constitutive activation of a mutant glycogen synthase. Biochimica et biophysica acta,

Hampl M, Eberhardt E, O'Reilly AO, Lampert A. 2016. Sodium channel slow inactivation interferes with open channel block SCIENTIFIC REPORTS, 6 >DOI >Link

Wang X, Wang R, Yang Y, Wu S, O'Reilly AO, Wu Y. 2016. A point mutation in the glutamate-gated chloride channel of Plutella xylostella is associated with resistance to abamectin INSECT MOLECULAR BIOLOGY, 25 :116-125 >DOI >Link

Amey JS, O'Reilly AO, Burton MJ, Puinean AM, Mellor IR, Duce IR, Field LM, Wallace BA, Williamson MS, Davies TGE. 2015. An evolutionarily-unique heterodimeric voltage-gated cation channel found in aphids FEBS LETTERS, 589 :598-607 >DOI >Link

Stadler T, O'Reilly AO, Lampert A. 2015. Erythromelalgia Mutation Q875E Stabilizes the Activated State of Sodium Channel Nav1.7 JOURNAL OF BIOLOGICAL CHEMISTRY, 290 :6316-6325 >DOI >Link

Schulze V, Stoetzer C, O'Reilly AO, Eberhardt E, Foadi N, Ahrens J, Wegner F, Lampert A, de la Roche J, Leffler A. 2014. The opioid methadone induces a local anaesthetic-like inhibition of the cardiac Na+ channel, Na(v)1.5 BRITISH JOURNAL OF PHARMACOLOGY, 171 :427-437 >DOI >Link

Eberhardt M, Nakajima J, Klinger AB, Neacsu C, Huehne K, O'Reilly AO, Kist AM, Lampe AK, Fischer K, Gibson J, Nau C, Winterpacht A, Lampert A. 2014. Inherited Pain SODIUM CHANNEL NAV1.7 A1632T MUTATION CAUSES ERYTHROMELALGIA DUE TO A SHIFT OF FAST INACTIVATION JOURNAL OF BIOLOGICAL CHEMISTRY, 289 :1971-1980 >DOI >Link

O'Reilly AO, Cole AR, Lopes JLS, Lampert A, Wallace BA. 2014. Chaperone-mediated native folding of a beta-scorpion toxin in the periplasm of Escherichia coli BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1840 :10-15 >DOI >Link

O'Reilly AO, Williamson MS, Gonzalez-Cabrera J, Turberg A, Field LM, Wallace BA, Davies TGE. 2014. Predictive 3D modelling of the interactions of pyrethroids with the voltage-gated sodium channels of ticks and mites PEST MANAGEMENT SCIENCE, 70 :369-377 >DOI >Link

O'Reilly AO, Eberhardt E, Weidner C, Alzheimer C, Wallace BA, Lampert A. 2012. Bisphenol A Binds to the Local Anesthetic Receptor Site to Block the Human Cardiac Sodium Channel PLOS ONE, 7 >DOI >Link

Erdmanis L, O'Reilly AO, Williamson MS, Field LM, Turberg A, Wallace BA. 2012. Association of Neonicotinoid Insensitivity with a Conserved Residue in the Loop D Binding Region of the Tick Nicotinic Acetylcholine Receptor BIOCHEMISTRY, 51 :4627-4629 >DOI >Link

Lampert A, O'Reilly AO, Reeh P, Leffler A. 2010. Sodium channelopathies and pain PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 460 :249-263 >DOI >Link

Powl AM, O apos Reilly AO, Miles AJ, Wallace BA. 2010. Synchrotron radiation circular dichroism spectroscopy-defined structure of the C-terminal domain of NaChBac and its role in channel assembly Proceedings of the National Academy of Sciences of the United States of America, 107 :14064-14069 >DOI

Charalambous K, O'Reilly AO, Bullough PA, Wallace BA. 2009. Thermal and chemical unfolding and refolding of a eukaryotic sodium channel Biochimica et Biophysica Acta - Biomembranes, 1788 :1279-1286 >DOI

Lampert A, O'Reilly AO, Dib-Hajj SD, Tyrrell L, Wallace BA, Waxman SG. 2008. A pore-blocking hydrophobic motif at the cytoplasmic aperture of the closed-state Nav1.7 channel is disrupted by the erythromelalgia- associated F1449V mutation Journal of Biological Chemistry, 283 :24118-24127 >DOI

Davies TGE, O'Reilly AO, Field LM, Wallace BA, Williamson MS. 2008. Knockdown resistance to DDT and pyrethroids: From target-site mutations to molecular modelling Pest Management Science, 64 :1126-1130 >DOI

Nurani G, Radford M, Charalambous K, O apos Reilly AO, Cronin NB, Haque S, Wallace BA. 2008. Tetrameric bacterial sodium channels: Characterization of structure, stability, and drug binding Biochemistry, 47 :8114-8121 >DOI

O'Reilly AO, Charalambous K, Nurani G, Powl AM, Wallace BA. 2008. G219S mutagenesis as a means of stabilizing conformational flexibility in the bacterial sodium channel NaChBac Molecular Membrane Biology, 25 :670-676 >DOI

Usherwood PNR, Davies TGE, Mellor IR, O'Reilly AO, Peng F, Vais H, Khambay BPS, Field LM, Williamson MS. 2007. Mutations in DIIS5 and the DIIS4-S5 linker of Drosophila melanogaster sodium channel define binding domains for pyrethroids and DDT FEBS Letters, 581 :5485-5492 >DOI

Bendahhou S, O'Reilly AO, Duclohier H. 2007. Role of hydrophobic residues in the voltage sensors of the voltage-gated sodium channel Biochimica et Biophysica Acta - Biomembranes, 1768 :1440-1447 >DOI

O'Reilly AO, Khambay BPS, Williamson MS, Field LM, Wallace BA, Davies TGE. 2006. Modelling insecticide-binding sites in the voltage-gated sodium channel Biochemical Journal, 396 :255-263 >DOI

Cronin NB, O'Reilly A, Duclohier H, Wallace BA. 2005. Effects of deglycosylation of sodium channels on their structure and function Biochemistry, 44 :441-449 >DOI

Cronin NB, O'Reilly A, Duclohier H, Wallace BA. 2003. Binding of the anticonvulsant drug lamotrigine and the neurotoxin batrachotoxin to voltage-gated sodium channels induces conformational changes associated with block and steady-state activation Journal of Biological Chemistry, 278 :10675-10682 >DOI

O'Reilly AO, Wallace BA. 2003. The peptaibol antiamoebin as a model ion channel: Similarities to bacterial potassium channels Journal of Peptide Science, 9 :769-775 >DOI

Conference Publication (journal proceedings)

Thull S, Naecsu C, O'Reilly A, Huth T, Meents J, Lampert A. 2016. Pyrethroid insecticide induces resurgent-like currents in human sodium channel Nav1.5 ACTA PHYSIOLOGICA, 216 >Link

Lampert A, O'Reilly A, Eberhardt E. 2013. Mutagenesis Studies Investigating Slow Inactivation of Sodium Channel Nav1.6 BIOPHYSICAL JOURNAL, 57th Annual Meeting of the Biophysical-Society 104 :134A-135A >Link

O'Reilly AO, Cole AR, Lampert A, Wallace BA. 2013. Functional Expression of a beta-Scorpion Toxin in the E. Coli Periplasm: A Tool for Exploring Sodium Channel/Ligand Interactions BIOPHYSICAL JOURNAL, 57th Annual Meeting of the Biophysical-Society 104 :401A-401A >Link

Lampert A, O'Reilly AO, Wallace BA, Alzheimer C. 2010. From Plastic-Bottle-Toxin to Sodium Channel Blocker: A New Role for Bisphenol A BIOPHYSICAL JOURNAL, 98 :114A-114A >Link