Liverpool Skylilne

Pharmacy and Biomolecular Sciences

Dr Alistair Fielding

Dr Alistair Fielding

Telephone: 0151 231 2576

Biography

Bruker Applications Scientist/Senior Lecturer (2012-2017), University of Manchester, UK
DFG Fellowship (2008-2012), Max-Planck for Biophysical Chemistry, Goettingen, Germany
CEA Fellowship (2006-2008) at CEA-Saclay, France
Postdoctoral research Associate (2003-2006), University of Denver, USA

RESEARCH INTERESTS
I am Senior Lecturer in Analytical Chemistry within the School of Pharmacy and Biomolecular Sciences. I have an established record in electron paramagnetic resonance (EPR) spectroscopy. On the recent move to LJMU my research will include further analytical techniques such as nuclear magnetic resonance (NMR) and mass spectroscopy as well as keeping strong links to the National EPR service. The Fielding group’s research is wide-ranging and problem driven.

Drug Interactions
A prime focus is using EPR and other spectroscopic techniques to measure drug interactions. A recent example can be found here: https://doi.org/10.1002/chem.201605099

Surface Interactions
Excitingly, we have developed a new synthetic strategy that uses spin labels to link biofunctional groups to surfaces: http://dx.doi.org/10.1002/anie.201703929

Structural Biology
We apply the latest EPR techniques to structural, mechanistic and kinetic questions concerning flavins, cytochromes, kinases and phosphatases. A good example of our work using double electron electron resonance (DEER) can be found here: http://dx.doi.org/10.1016/j.str.2016.10.006
Other expertise includes UV-vis stopped flow and rapid freeze-quench.

Materials Science
We have a number of collaborations concerning novel materials. We have recently worked on the surface chemistry of MoS2 using EPR: http://dx.doi.org/10.1021/acs.chemmater.7b01245

In all endeavours, we welcome collaborative partners both nationally and abroad. Phone calls always welcome.

Industrial consulting in EPR spectroscopy, possible.

We work with several industrial companies concerning challenging analytical problems. We welcome enquiries.

PhD Students
Currently, four students have gained their PhD under my supervision. All have gone on to careers in either academia or industry. New applications are always welcome.

Teaching
I teach analytical and physical chemistry. Topics include: NMR, EPR, AAS, ICP-OES, ICP-MS, IR, UV-vis, Electrochemistry, Group theory, Hückel molecular orbital theory.

Publications

Highlighted publications

Gonzalez J, Alcantara R, Tirado JL, Fielding AJ, Dryfe R. 2017. Electrochemical Interaction of Few-layer Molybdenum Disulphide Composites vs Sodium: New Insights on the Reaction Mechanism Chemistry of Materials, 29 :5886-5895 >DOI

Hollas MA, Webb SJ, Flitsch SL, Fielding AJ. 2017. A Bifunctional Spin Label for Ligand Recognition on Surfaces Angewandte Chemie International Edition, 56 :9449-9453 >DOI

Fielding AJ, Evans PG, Alizadeh S, Bisby R, Drew MGB, Del Casino A, Dunn JF, Randle LE, Dempster NM, Nahar L, Sarker SD, Cantú Reinhard FG, de Visser SP, Dascombe MJ, Ismail FMD. 2017. Modulation of Antimalarial Activity at a Putative Bisquinoline Receptor in vivo Using Fluorinated Bisquinolines Chemistry - A European Journal, >DOI >Link

Journal article

Del Casino A, Valentina L, Rakesh B, Randle LE, Dascombe MJ, Fennell DBJ, Drew MGB, Angus B, Fielding AJ, Ismail FMD. 2018. Synthesis, Structural Determination, and Pharmacology of Putative Dinitroaniline Antimalarials ChemistrySelect, 3 :7572-7580 >DOI

Moro F, Bhuiyan MA, Kudrynskyi ZR, Puttock R, Kazakova O, Makarovsky O, Fay MW, Parmenter C, Kovalyuk ZD, Fielding AJ, Kern M, van Slageren J, Patanè A. 2018. Room Temperature Uniaxial Magnetic Anisotropy Induced By Fe‐Islands in the InSe Semiconductor Van Der Waals Crystal Advanced Science, >DOI

Brownbill NJ, Sprick RS, Bonillo B, Pawsey S, Aussenac F, Fielding AJ, Cooper AI, Blanc F. 2018. Structural Elucidation of Amorphous Photocatalytic Polymers from Dynamic Nuclear Polarization Enhanced Solid State NMR Macromolecules, 51 :3088-3096 >DOI >Link

Wang B, Fielding AJ, Dryfe RAW. 2018. In situ electrochemical electron paramagnetic resonance spectroscopy as a tool to probe electrical double layer capacitance Chemical Communications, 54 :3827-3830 >DOI

Boulon ME, Fernandez A, Moreno Pineda E, Chilton NF, Timco G, Fielding AJ, Winpenny REP. 2017. Measuring Spin⋅⋅⋅Spin Interactions between Heterospins in a Hybrid [2]Rotaxane Angewandte Chemie International Edition, 56 :3876-3879 >DOI

Park SS, Hendon CH, Fielding AJ, Walsh A, O'Keeffe M, Dinca M. 2017. The Organic Secondary Building Unit: Strong Intermolecular pi Interactions Define Topology in MIT-25, a Mesoporous MOF with Proton-Replete Channels JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 139 :3619-3622 >DOI >Link

Gonzalez J, Alcantara R, Tirado JL, Fielding AJ, Dryfe R. 2017. Electrochemical Interaction of Few-layer Molybdenum Disulphide Composites vs Sodium: New Insights on the Reaction Mechanism Chemistry of Materials, 29 :5886-5895 >DOI

Brandariz-de-Pedro G, Heyes DJ, Hardman SJO, Shanmugam M, Jones AR, Weber S, Nohr D, Scrutton NS, Fielding AJ. 2017. Direct Evidence of an Excited-State Triplet Species upon Photoactivation of the Chlorophyll Precursor Protochlorophyllide JOURNAL OF PHYSICAL CHEMISTRY LETTERS, 8 :1219-1223 >DOI >Link

Hollas MA, Webb SJ, Flitsch SL, Fielding AJ. 2017. A Bifunctional Spin Label for Ligand Recognition on Surfaces Angewandte Chemie International Edition, 56 :9449-9453 >DOI

Fielding AJ, Evans PG, Alizadeh S, Bisby R, Drew MGB, Del Casino A, Dunn JF, Randle LE, Dempster NM, Nahar L, Sarker SD, Cantú Reinhard FG, de Visser SP, Dascombe MJ, Ismail FMD. 2017. Modulation of Antimalarial Activity at a Putative Bisquinoline Receptor in vivo Using Fluorinated Bisquinolines Chemistry - A European Journal, >DOI >Link

Concilio MG, Fielding AJ, Bayliss R, Burgess SG. 2016. Density functional theory studies of MTSL nitroxide side chain conformations attached to an activation loop Theoretical Chemistry Accounts, 135 >DOI

Moro F, Turyanska L, Wilman J, Williams HEL, Fielding AJ, Patanè A. 2016. Surface Sensing of Quantum Dots by Electron Spins Nano Letters, 16 :6343-6348 >DOI

Gahloth D, Levy C, Heaven G, Stefani F, Wunderley L, Mould P, Cliff MJ, Bella J, Fielding AJ, Woodman P, Tabernero L. 2016. Structural Basis for Selective Interaction between the ESCRT Regulator HD-PTP and UBAP1 Structure, 24 :2115-2126 >DOI

Parey K, Fielding AJ, Sörgel M, Rachel R, Huber H, Ziegler C, Rajendran C. 2016. In meso crystal structure of a novel membrane-associated octaheme cytochrome c from the Crenarchaeon Ignicoccus hospitalis The FEBS Journal, 283 :3807-3820 >DOI

Kwon H, Basran J, Casadei CM, Fielding AJ, Schrader TE, Ostermann A, Devos JM, Aller P, Blakeley MP, Moody PCE, Raven EL. 2016. Direct visualization of a Fe(IV)–OH intermediate in a heme enzyme Nature Communications, 7 :13445-13445 >DOI

Burgess SG, Grazia Concilio M, Bayliss R, Fielding AJ. 2016. Detection of Ligand-induced Conformational Changes in the Activation Loop of Aurora-A Kinase by PELDOR Spectroscopy ChemistryOpen, 5 :531-534 >DOI

Loughrey JJ, Patmore NJ, Baldansuren A, Fielding AJ, McInnes EJL, Hardie MJ, Sproules S, Halcrow MA. 2015. Platinum( ii ) complexes of mixed-valent radicals derived from cyclotricatechylene, a macrocyclic tris-dioxolene Chemical Science, 6 :6935-6948 >DOI

Moro F, Turyanska L, Wilman J, Fielding AJ, Fay MW, Granwehr J, Patanè A. 2015. Electron spin coherence near room temperature in magnetic quantum dots Scientific Reports, 5 >DOI

Murray AT, Dowley MJH, Pradaux-Caggiano F, Baldansuren A, Fielding AJ, Tuna F, Hendon CH, Walsh A, Lloyd-Jones GC, John MP, Carbery DR. 2015. Catalytic Amine Oxidation under Ambient Aerobic Conditions: Mimicry of Monoamine Oxidase B Angewandte Chemie International Edition, 54 :8997-9000 >DOI

Ardavan A, Bowen AM, Fernandez A, Fielding AJ, Kaminski D, Moro F, Muryn CA, Wise MD, Ruggi A, McInnes EJL, Severin K, Timco GA, Timmel CR, Tuna F, Whitehead GFS, Winpenny REP. 2015. Engineering coherent interactions in molecular nanomagnet dimers npj Quantum Information, 1 >DOI

Fielding A, Concilio M, Heaven G, Hollas M. 2014. New Developments in Spin Labels for Pulsed Dipolar EPR Molecules, 19 :16998-17025 >DOI

Casadei CM, Gumiero A, Metcalfe CL, Murphy EJ, Basran J, Concilio MG, Teixeira SCM, Schrader TE, Fielding AJ, Ostermann A, Blakeley MP, Raven EL, Moody PCE. 2014. Neutron cryo-crystallography captures the protonation state of ferryl heme in a peroxidase Science, 345 :193-197 >DOI

Koch C, Tria G, Fielding AJ, Brodhun F, Valerius O, Feussner K, Braus GH, Svergun DI, Bennati M, Feussner I. 2013. A structural model of PpoA derived from SAXS-analysis—Implications for substrate conversion Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1831 :1449-1457 >DOI

Fielding AJ, Parey K, Ermler U, Scheller S, Jaun B, Bennati M. 2013. Advanced electron paramagnetic resonance on the catalytic iron–sulfur cluster bound to the CCG domain of heterodisulfide reductase and succinate: quinone reductase JBIC Journal of Biological Inorganic Chemistry, 18 :905-915 >DOI

Pudney CR, Lane RSK, Fielding AJ, Magennis SW, Hay S, Scrutton NS. 2013. Enzymatic Single-Molecule Kinetic Isotope Effects Journal of the American Chemical Society, 135 :3855-3864 >DOI

Pievo R, Angerstein B, Fielding AJ, Koch C, Feussner I, Bennati M. 2013. A Rapid Freeze-Quench Setup for Multi-Frequency EPR Spectroscopy of Enzymatic Reactions ChemPhysChem, 14 :4094-4101 >DOI

Koch C, Fielding AJ, Brodhun F, Bennati M, Feussner I. 2012. Linoleic acid positioning in psi factor producing oxygenase A, a fusion protein with an atypical cytochrome P450 activity FEBS Journal, 279 :1594-1606 >DOI

Fielding AJ, Brodhun F, Koch C, Pievo R, Denysenkov V, Feussner I, Bennati M. 2011. Multifrequency Electron Paramagnetic Resonance Characterization of PpoA, a CYP450 Fusion Protein that Catalyzes Fatty Acid Dioxygenation Journal of the American Chemical Society, 133 :9052-9062 >DOI

Belin P, Le Du MH, Fielding A, Lequin O, Jacquet M, Charbonnier J-B, Lecoq A, Thai R, Courcon M, Masson C, Dugave C, Genet R, Pernodet J-L, Gondry M. 2009. Identification and structural basis of the reaction catalyzed by CYP121, an essential cytochrome P450 in Mycobacterium tuberculosis Proceedings of the National Academy of Sciences, 106 :7426-7431 >DOI

Usselman RJ, Fielding AJ, Frerman FE, Watmough NJ, Eaton GR, Eaton SS. 2008. Impact of Mutations on the Midpoint Potential of the [4Fe-4S] +1,+2 Cluster and on Catalytic Activity in Electron Transfer Flavoprotein-ubiquinone Oxidoreductase (ETF-QO) † Biochemistry, 47 :92-100 >DOI

Fielding AJ, Usselman RJ, Watmough N, Simkovic M, Frerman FE, Eaton GR, Eaton SS. 2008. Electron spin relaxation enhancement measurements of interspin distances in human, porcine, and Rhodobacter electron transfer flavoprotein–ubiquinone oxidoreductase (ETF–QO) Journal of Magnetic Resonance, 190 :222-232 >DOI

Fielding AJ, Singh R, Boscolo B, Loewen PC, Ghibaudi EM, Ivancich A. 2008. Intramolecular Electron Transfer versus Substrate Oxidation in Lactoperoxidase: Investigation of Radical Intermediates by Stopped-Flow Absorption Spectrophotometry and (9−285 GHz) Electron Paramagnetic Resonance Spectroscopy Biochemistry, 47 :9781-9792 >DOI

Sato H, Kathirvelu V, Fielding A, Blinco JP, Micallef AS, Bottle SE, Eaton SS, Eaton GR. 2007. Impact of molecular size on electron spin relaxation rates of nitroxyl radicals in glassy solvents between 100 and 300 K Molecular Physics, 105 :2137-2151 >DOI

Fielding AJ, Fox S, Millhauser GL, Chattopadhyay M, Kroneck PM, Fritz G, Eaton GR, Eaton SS. 2006. Electron spin relaxation of copper(II) complexes in glassy solution between 10 and 120K Journal of Magnetic Resonance, 179 :92-104 >DOI

Fielding AJ, Carl PJ, Eaton GR, Eaton SS. 2005. Multifrequency EPR of four triarylmethyl radicals Applied Magnetic Resonance, 28 :231-238 >DOI

Engagement & Impact

Industrial connections:

AstraZeneca, Alderley Park (15/09/16), Bespoke talk titled: Electron Paramagnetic Resonance Spectroscopy in Pharmaceutical Research, Development and Analysis

Membership of professional bodies:

Member of the International EPR Society, International EPR Society

Member of the Royal Society of Chemistry, Royal Society of Chemistry

Other Professional Activity:

Honorary Lecturer, The University of Manchester

Teaching qualification:

HEA Fellowship