Liverpool Skylilne

Pharmacy and Biomolecular Sciences

Dr Alistair Fielding

Dr Alistair Fielding

Telephone: 0151 231 2576

ORCID

Biography

Bruker Applications Scientist/Senior Lecturer (2012-2017), University of Manchester, UK
DFG Fellowship (2008-2012), Max-Planck for Biophysical Chemistry, Goettingen, Germany
CEA Fellowship (2006-2008) at CEA-Saclay, France
Postdoctoral research Associate (2003-2006), University of Denver, USA

RESEARCH INTERESTS
I am Senior Lecturer in Analytical Chemistry within the School of Pharmacy and Biomolecular Sciences. The Fielding group uses electron paramagnetic resonance (EPR) spectroscopy to solve biological and material science problems concerning structure and transient intermediates. We also use further analytical techniques such as nuclear magnetic resonance (NMR) and mass spectroscopy. The Fielding group’s research is wide-ranging and problem driven.

Plastic Waste
We have recently successfully obtained a Leverhulme Fellowship (2019-2020) in a project titled: “Understanding the radical source of the degradation of plastic waste”. The project aims to achieve a comprehensive understanding of the radical mechanism of action involved in the degradation of common plastic waste materials. We have done several media appearances on this subject and are happy to do more.

Drug Interactions
A prime focus is using EPR and other spectroscopic techniques to measure drug interactions. A recent example can be found is: Fielding, A.J.* et al. Chemistry – A European Journal 2017, 23, 6811-6828

Surface Interactions
Excitingly, we have developed a new synthetic strategy that uses spin labels to link biofunctional groups to surfaces (Fielding, A.J.* et al., A Bifunctional Spin Label for Ligand Recognition on Surfaces, Angewandte Chemie - International Edition 2017, 56, 9449-9453).

Structural Biology
We apply the latest EPR techniques to structural, mechanistic and kinetic questions concerning flavins, cytochromes, kinases and phosphatases. A good example of our work using double electron electron resonance (DEER): Fielding, A.J. et al., Structure 2016, 24, 2115–2126.

Materials Science
We have a number of collaborations concerning novel materials. We have recently worked on the surface chemistry of MoS2 using EPR (Fielding, A.J.* et al., Chemistry of Materials 2017, 29, 5886-5895).

Other expertise includes UV-vis stopped flow and rapid freeze-quench.

In all endeavours, we welcome collaborative partners both nationally and abroad. Phone calls always welcome.

Industrial consulting in EPR spectroscopy, possible.

We work with several industrial companies concerning challenging analytical problems. We welcome enquiries.

We can be found on twitter: @Fielding_Group

PhD Students
Currently, six students have gained their PhD under my supervision. All have gone on to careers in either academia or industry. New applications are always welcome.

Teaching
I teach analytical and physical chemistry. Topics include: NMR, EPR, AAS, ICP-OES, ICP-MS, IR, UV-vis, electrochemistry, Group Theory and transition metal spectroscopy.

Publications

Highlighted publications

Wang B, Fielding AJ, Dryfe RAW. 2018. Electron Paramagnetic Resonance Investigation of the Structure of Graphene Oxide: pH-Dependence of the Spectroscopic Response ACS Applied Nano Materials, 2 :19-27 DOI Publisher Url Public Url

Hollas MA, Webb SJ, Flitsch SL, Fielding AJ. 2017. A Bifunctional Spin Label for Ligand Recognition on Surfaces Angewandte Chemie International Edition, 56 :9449-9453 DOI Author Url Publisher Url Public Url

Fielding AJ, Evans PG, Alizadeh S, Bisby R, Drew MGB, Del Casino A, Dunn JF, Randle LE, Dempster NM, Nahar L, Sarker SD, Cantú Reinhard FG, de Visser SP, Dascombe MJ, Ismail FMD. 2017. Modulation of Antimalarial Activity at a Putative Bisquinoline Receptor in vivo Using Fluorinated Bisquinolines Chemistry - A European Journal, DOI Author Url Public Url

Journal article

Freeman SL, Kwon H, Portolano N, Parkin G, Girija UV, Basran J, Fielding AJ, Fairall L, Svistunenko DA, Moody PCE, Schwabe JWR, Kyriacou CP, Raven EL. 2019. Heme binding to human CLOCK affects interactions with the E-box PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 116 :19911-19916 DOI Author Url Public Url

Lockyer SJ, Fielding AJ, Whitehead GFS, Timco GA, Winpenny REP, McInnes EJL. 2019. Close Encounters of the Weak Kind: Investigations of Electron-Electron Interactions between Dissimilar Spins in Hybrid Rotaxanes JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 141 :14633-14642 DOI Author Url Public Url

Wang B, Fielding AJ, Dryfe RAW. 2019. Electron Paramagnetic Resonance as a Structural Tool to Study Graphene Oxide: Potential-Dependence of the EPR Response The Journal of Physical Chemistry C, DOI Author Url Publisher Url Public Url

Fernandez-Soto A, Bruce A, Fielding A, Cavet J, Tabernero L. 2019. Mechanism of catalysis and inhibition of Mycobacterium tuberculosis SapM, implications for the development of novel antivirulence drugs. Scientific Reports, DOI Author Url Public Url

Moro F, Fielding A, Turyanska L, Patanè A. 2019. Realization of Universal Quantum Gates with Spin-Qudits in Colloidal Quantum Dots Advanced Quantum Technologies, DOI Public Url

Lukinović V, Woodward JR, Marrafa TC, Shanmugam M, Heyes DJ, Hardman SJO, Scrutton NS, Hay S, Fielding A, Jones AR. 2019. The Photochemical Spin Dynamics of the Vitamin B12 Derivative, Methylcobalamin The Journal of Physical Chemistry Part B: Condensed Matter, Materials, Surfaces, Interfaces and Biophysical, DOI Author Url Publisher Url Public Url

Wang B, Fielding AJ, Dryfe RAW. 2018. Electron Paramagnetic Resonance Investigation of the Structure of Graphene Oxide: pH-Dependence of the Spectroscopic Response ACS Applied Nano Materials, 2 :19-27 DOI Publisher Url Public Url

Del Casino A, Valentina L, Rakesh B, Randle LE, Dascombe MJ, Fennell DBJ, Drew MGB, Angus B, Fielding AJ, Ismail FMD. 2018. Synthesis, Structural Determination, and Pharmacology of Putative Dinitroaniline Antimalarials ChemistrySelect, 3 :7572-7580 DOI Author Url Publisher Url Public Url

Moro F, Bhuiyan MA, Kudrynskyi ZR, Puttock R, Kazakova O, Makarovsky O, Fay MW, Parmenter C, Kovalyuk ZD, Fielding AJ, Kern M, van Slageren J, Patanè A. 2018. Room Temperature Uniaxial Magnetic Anisotropy Induced By Fe‐Islands in the InSe Semiconductor Van Der Waals Crystal Advanced Science, DOI Publisher Url Public Url

Brownbill NJ, Sprick RS, Bonillo B, Pawsey S, Aussenac F, Fielding AJ, Cooper AI, Blanc F. 2018. Structural Elucidation of Amorphous Photocatalytic Polymers from Dynamic Nuclear Polarization Enhanced Solid State NMR Macromolecules, 51 :3088-3096 DOI Author Url Publisher Url Public Url

Wang B, Fielding AJ, Dryfe RAW. 2018. In situ electrochemical electron paramagnetic resonance spectroscopy as a tool to probe electrical double layer capacitance Chemical Communications, 54 :3827-3830 DOI Author Url Publisher Url Public Url

Gonzalez J, Alcantara R, Tirado JL, Fielding AJ, Dryfe R. 2017. Electrochemical Interaction of Few-layer Molybdenum Disulphide Composites vs Sodium: New Insights on the Reaction Mechanism Chemistry of Materials, 29 :5886-5895 DOI Author Url Publisher Url Public Url

Hollas MA, Webb SJ, Flitsch SL, Fielding AJ. 2017. A Bifunctional Spin Label for Ligand Recognition on Surfaces Angewandte Chemie International Edition, 56 :9449-9453 DOI Author Url Publisher Url Public Url

Fielding AJ, Evans PG, Alizadeh S, Bisby R, Drew MGB, Del Casino A, Dunn JF, Randle LE, Dempster NM, Nahar L, Sarker SD, Cantú Reinhard FG, de Visser SP, Dascombe MJ, Ismail FMD. 2017. Modulation of Antimalarial Activity at a Putative Bisquinoline Receptor in vivo Using Fluorinated Bisquinolines Chemistry - A European Journal, DOI Author Url Public Url

Boulon ME, Fernandez A, Moreno Pineda E, Chilton NF, Timco G, Fielding AJ, Winpenny REP. 2017. Measuring Spin⋅⋅⋅Spin Interactions between Heterospins in a Hybrid [2]Rotaxane Angewandte Chemie International Edition, 56 :3876-3879 DOI Author Url Publisher Url Public Url

Brandariz-de-Pedro G, Heyes DJ, Hardman SJO, Shanmugam M, Jones AR, Weber S, Nohr D, Scrutton NS, Fielding AJ. 2017. Direct Evidence of an Excited-State Triplet Species upon Photoactivation of the Chlorophyll Precursor Protochlorophyllide JOURNAL OF PHYSICAL CHEMISTRY LETTERS, 8 :1219-1223 DOI Author Url Public Url

Park SS, Hendon CH, Fielding AJ, Walsh A, O'Keeffe M, Dinca M. 2017. The Organic Secondary Building Unit: Strong Intermolecular pi Interactions Define Topology in MIT-25, a Mesoporous MOF with Proton-Replete Channels Journal of the American Chemical Society, 139 :3619-3622 DOI Author Url Publisher Url Public Url

Gahloth D, Levy C, Heaven G, Stefani F, Wunderley L, Mould P, Cliff MJ, Bella J, Fielding AJ, Woodman P, Tabernero L. 2016. Structural Basis for Selective Interaction between the ESCRT Regulator HD-PTP and UBAP1 Structure, 24 :2115-2126 DOI Public Url

Burgess SG, Grazia Concilio M, Bayliss R, Fielding AJ. 2016. Detection of Ligand-induced Conformational Changes in the Activation Loop of Aurora-A Kinase by PELDOR Spectroscopy ChemistryOpen, 5 :531-534 DOI Author Url Publisher Url Public Url

Kwon H, Basran J, Casadei CM, Fielding AJ, Schrader TE, Ostermann A, Devos JM, Aller P, Blakeley MP, Moody PCE, Raven EL. 2016. Direct visualization of a Fe(IV)–OH intermediate in a heme enzyme Nature Communications, 7 DOI Publisher Url Public Url

Moro F, Turyanska L, Wilman J, Williams HEL, Fielding AJ, Patanè A. 2016. Surface Sensing of Quantum Dots by Electron Spins Nano Letters, 16 :6343-6348 DOI Publisher Url Public Url

Loughrey JJ, Patmore NJ, Baldansuren A, Fielding AJ, McInnes EJL, Hardie MJ, Sproules S, Halcrow MA. 2015. Platinum( ii ) complexes of mixed-valent radicals derived from cyclotricatechylene, a macrocyclic tris-dioxolene Chemical Science, 6 :6935-6948 DOI Publisher Url Public Url

Ardavan A, Bowen AM, Fernandez A, Fielding AJ, Kaminski D, Moro F, Muryn CA, Wise MD, Ruggi A, McInnes EJL, Severin K, Timco GA, Timmel CR, Tuna F, Whitehead GFS, Winpenny REP. 2015. Engineering coherent interactions in molecular nanomagnet dimers npj Quantum Information, 1 DOI Public Url

Moro F, Turyanska L, Wilman J, Fielding AJ, Fay MW, Granwehr J, Patanè A. 2015. Electron spin coherence near room temperature in magnetic quantum dots Scientific Reports, 5 DOI Publisher Url Public Url

Murray AT, Dowley MJH, Pradaux-Caggiano F, Baldansuren A, Fielding AJ, Tuna F, Hendon CH, Walsh A, Lloyd-Jones GC, John MP, Carbery DR. 2015. Catalytic Amine Oxidation under Ambient Aerobic Conditions: Mimicry of Monoamine Oxidase B Angewandte Chemie International Edition, 54 :8997-9000 DOI Public Url

Fielding A, Concilio M, Heaven G, Hollas M. 2014. New Developments in Spin Labels for Pulsed Dipolar EPR Molecules, 19 :16998-17025 DOI Public Url

Casadei CM, Gumiero A, Metcalfe CL, Murphy EJ, Basran J, Concilio MG, Teixeira SCM, Schrader TE, Fielding AJ, Ostermann A, Blakeley MP, Raven EL, Moody PCE. 2014. Neutron cryo-crystallography captures the protonation state of ferryl heme in a peroxidase Science, 345 :193-197 DOI Publisher Url Public Url

Pievo R, Angerstein B, Fielding AJ, Koch C, Feussner I, Bennati M. 2013. A Rapid Freeze-Quench Setup for Multi-Frequency EPR Spectroscopy of Enzymatic Reactions ChemPhysChem, 14 :4094-4101 DOI

Pudney CR, Lane RSK, Fielding AJ, Magennis SW, Hay S, Scrutton NS. 2013. Enzymatic Single-Molecule Kinetic Isotope Effects Journal of the American Chemical Society, 135 :3855-3864 DOI

Fielding AJ, Brodhun F, Koch C, Pievo R, Denysenkov V, Feussner I, Bennati M. 2011. Multifrequency Electron Paramagnetic Resonance Characterization of PpoA, a CYP450 Fusion Protein that Catalyzes Fatty Acid Dioxygenation Journal of the American Chemical Society, 133 :9052-9062 DOI

Belin P, Le Du MH, Fielding A, Lequin O, Jacquet M, Charbonnier J-B, Lecoq A, Thai R, Courcon M, Masson C, Dugave C, Genet R, Pernodet J-L, Gondry M. 2009. Identification and structural basis of the reaction catalyzed by CYP121, an essential cytochrome P450 in Mycobacterium tuberculosis Proceedings of the National Academy of Sciences, 106 :7426-7431 DOI

Fielding AJ, Singh R, Boscolo B, Loewen PC, Ghibaudi EM, Ivancich A. 2008. Intramolecular Electron Transfer versus Substrate Oxidation in Lactoperoxidase: Investigation of Radical Intermediates by Stopped-Flow Absorption Spectrophotometry and (9−285 GHz) Electron Paramagnetic Resonance Spectroscopy Biochemistry, 47 :9781-9792 DOI

Sato H, Kathirvelu V, Fielding A, Blinco JP, Micallef AS, Bottle SE, Eaton SS, Eaton GR. 2007. Impact of molecular size on electron spin relaxation rates of nitroxyl radicals in glassy solvents between 100 and 300 K Molecular Physics, 105 :2137-2151 DOI

Parey K, Fielding AJ, Sörgel M, Rachel R, Huber H, Ziegler C, Rajendran C. In meso crystal structure of a novel membrane-associated octaheme cytochrome c from the Crenarchaeon Ignicoccus hospitalis The FEBS Journal, 283 :3807-3820 DOI Public Url

Fielding AJ, Parey K, Ermler U, Scheller S, Jaun B, Bennati M. Advanced electron paramagnetic resonance on the catalytic iron–sulfur cluster bound to the CCG domain of heterodisulfide reductase and succinate: quinone reductase JBIC Journal of Biological Inorganic Chemistry, 18 :905-915 DOI

Koch C, Tria G, Fielding AJ, Brodhun F, Valerius O, Feussner K, Braus GH, Svergun DI, Bennati M, Feussner I. A structural model of PpoA derived from SAXS-analysis—Implications for substrate conversion Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1831 :1449-1457 DOI

Koch C, Fielding AJ, Brodhun F, Bennati M, Feussner I. Linoleic acid positioning in psi factor producing oxygenase A, a fusion protein with an atypical cytochrome P450 activity FEBS Journal, 279 :1594-1606 DOI

Concilio MG, Fielding AJ, Bayliss R, Burgess SG. Density functional theory studies of MTSL nitroxide side chain conformations attached to an activation loop Theoretical Chemistry Accounts, 135 DOI Public Url

Fielding AJ, Usselman RJ, Watmough N, Simkovic M, Frerman FE, Eaton GR, Eaton SS. Electron spin relaxation enhancement measurements of interspin distances in human, porcine, and Rhodobacter electron transfer flavoprotein–ubiquinone oxidoreductase (ETF–QO) Journal of Magnetic Resonance, 190 :222-232 DOI

Usselman RJ, Fielding AJ, Frerman FE, Watmough NJ, Eaton GR, Eaton SS. Impact of Mutations on the Midpoint Potential of the [4Fe-4S]+1,+2Cluster and on Catalytic Activity in Electron Transfer Flavoprotein-ubiquinone Oxidoreductase (ETF-QO)† Biochemistry, 47 :92-100 DOI

Fielding AJ, Fox S, Millhauser GL, Chattopadhyay M, Kroneck PMH, Fritz G, Eaton GR, Eaton SS. Electron spin relaxation of copper(II) complexes in glassy solution between 10 and 120K Journal of Magnetic Resonance, 179 :92-104 DOI

Fielding AJ, Carl PJ, Eaton GR, Eaton SS. Multifrequency EPR of four triarylmethyl radicals Applied Magnetic Resonance, 28 :231-238 DOI

Engagement & Impact

Industrial connections:

AstraZeneca, Alderley Park (15/09/16), Bespoke talk titled: Electron Paramagnetic Resonance Spectroscopy in Pharmaceutical Research, Development and Analysis. 2015

Teaching qualification:

HEA Fellowship. 2015

Other Professional Activity:

Honorary Lecturer, The University of Manchester.

Honorary Research Associate, Institute of Ageing and Chronic Disease, University of Liverpool.

Membership of professional bodies:

Member of the International EPR Society, International EPR Society.

Member of the Royal Society of Chemistry, Royal Society of Chemistry.