Andrew's research interests include:
1. Developing molecular interaction, activity and detection/quantification assays.
2. Understanding the action of proteins (cytokines, growth factors, bacterial adhesins) and charged carbohydrates that mediate cell growth, differentiation, migration, adhesion and innate immunity.
3. Chromatographic purification and analysis of charged animal polysaccharides and oligosaccharides.
Andrew currently uses:
Plate reader absorbance/fluorescence or immuno assays,
Differential scanning fluorimetry protein thermostability assays,
Cell line/primary cell response assays (MTT, Q-PCR, ELISA), flow cytometry and microscopy,
He collaborates with cell biologists, synthetic chemists and biophysicists.
Andrew is interested in enquiries from PhD or MPhil candidates who have funding. Please email him to enquire.
A summary of his research history, outside engagement and teaching is below and under other tabs on this website.
Andrew obtained an MBiochem honours undergraduate degree in Biochemistry from the University of Oxford. During this he completed a 4 month research project in the Glycobiology Institute, developing and exploiting novel methods in mass spectrometric analysis of negatively-charged, sialylated N-glycans, published as 2 papers with over 320 citations.
Andrew then spent a further year at the Institute learning protein expression/biochemistry to investigate the oligomeric macro-structure of the asialoglycoprotein receptor that mediates liver endocytosis.
He left Oxford to begin an MRC funded PhD at the University of Birmingham. Here he investigated the fibroblast growth factor signalling system. This involved receptor/ligand protein expression, bioconjugation labelling, innovating ELISA interaction methods and using them with optical biosensor methodology (Biacore surface plasmon resonance and IAsys resonant mirror) or cellular MTT assays to characterise interaction specificity, kinetics and ligand-receptor-heparan sulfate ternary complex assembly. He also critically reviewed methodology for investigating heparan sulfate carbohydrates and their interactions with proteins. Several publications were produced, now with approaching 1000 citations, collectively.
To improve progress in structure-function studies, Andrew begun developing a glycoarray immunoassay screening platform, via a BBSRC grant. At the same time, he helped write a patent on a method for creating highly diverse heparin libraries for drug discovery. Heparins are members of the heparan sulfate family and are natural product pharmaceuticals used to prevent blood clotting ($8 billion market). They are also known bind/regulate 100-1000s of extracellular proteins linked to inflammation, infection, cancer, neurodegeneration and regenerative biology, hence are a rich resource for drug discovery. To exploit the technology, Andrew and others founded IntelliHep Ltd and reached the final of the 2001 BBSRC business plan competition. The team transferred to the University of Liverpool, where Andrew received the award for the 2005 BioNow Bioentrepreneur of the Year, for his work on IntelliHep Ltd.
At the University of Liverpool, via a subsequent BBSRC grant, Andrew tested the first generation glycoarrays technology for screening saccharide libraries, alongside competitive ELISA and optical biosensor screens. He later joined the UK glycoarrays consortium, that consisted of research groups from Imperial, Oxford Manchester, Dundee, East Anglia and Liverpool Universities, developing a second generation gold surface-assembled monolayer array platform, compatible with all carbohydrates and fluorescence immunoassay, SPR and mass spectrometry read-outs. Andrew also produced saccharide libraries, using chemical/enzymatic cleavage and orthogonal chromatographic methods, for use in protein interaction screening, Slit-Robo receptor/ligand complex formation assays and developing mass spectrometry structural analysis. Together this work has gained approaching 600 citations. Then, as Director of IntelliHep Ltd, he raised funding and managed two heparin-based saccharide bioprocessing pilot projects at the National Biomanufacturing Centre (that latter became Allergan Biologics), for which he was awarded runner-up for the 2008 BioNow Emerging Technology of the year award.
Andrew secured a lectureship at Liverpool John Moores University and later became part of the UK glycoarrays consortium steering committee, being part of a consortium EPSRC grant led by the University of Manchester and supervising a PDRA at the University of Liverpool. Andrew continued collaborations developing glycoarray cellular assay methodology, analysing HS-antibody interactions using ELISAs and the Slit-Robo/heparan sulfate signalling system leading to papers that now have 500+ citations, collectively.
Since 2008, Andrew has also developed independent research and gained funding from UKRI and charity sources on projects listed at the top. He remained Director of IntelliHep Ltd, who sought to secure funding to exploit their general know-how in heparin drug discovery and specific patents in Alzheimer Disease, cancer metastasis and spinal repair.
Andrew teaches protein and carbohydrate structure, purification, analysis and interactions at undergraduate and masters level with a focus on technical approaches, real-world context and employability. His teaching approach is heavily focussed on constructive alignment of learning outcomes, teaching activities and assessment. As a result, he uses active learning to promote deeper learning to supplement live-annotation of powerpoints to deliver lecture material. He has received numerous nominations/shortlistings for university teaching, personal tutor, project supervisor and employability awards from students.
Andrew has trained undergraduate project students in extraction, purification and analytical chromatography, as well as interaction assays and the use of protein bioinformatics, helping them gain graduate/doctoral study (e.g. at Imperial and Manchester Universities) and positions in the analytical industry.
1) Develop and use methods/assays to:
a) Characterise biomolecular interactions and structure-function relationships.
b) Purify and Analyse biomolecules.
c) Detect and Quantify biomolecules.
a) Macromolecule drug development and monitoring.
b) Diagnostic/prognostic test development.
To date, Andrew has mostly focussed on proteins and carbohydrates. E.g.:
a) Cell signalling proteins and their receptors.
b) Microbial adhesion
c) Sulfated carbohydrates from the heparin/heparan sulphate family.
He has supervised ~ 3 postdoctoral researchers,10 masters/PhD students, 5 summer students/interns, 65 honours project students.
Andrew is coFounder and director of IntelliHep Ltd, a heparin therapuetics spin-out company.
Using equipment available in LJMU Faculty of Science: multiple BMG and Tecan plate readers (absorbance, fluorescence intensity, fluorescence polarisation, time-resolved fluorescence, CO2 incubation), BD flow cytometry, Leica confocal and cell incubation microscopes, Qiagen Q-PCR, numerous HPLCs and cell culture suites.
His research has been funded by the EPSRC, BBSRC, MRC, Rosetrees Trust, Biochemical Society and LJMU.
F) INTERESTS SUMMARY
- Method Development (immunoassays, plate reader assays, optical biosensors, microarrays, bioassays, chromatography).
- Analytical Biochemistry (molecular interactions, structure-function relationships, macromolecule purification & analysis).
- Cell Biology (cell signaling, microbial adhesion, homeostasis & immunity of epithelia, blood coagulation).
- Proteins (e.g. growth factors, cytokines, chemokines, proteases)
- Carbohydrates (e.g. heparin/heparan sulfate/glycosaminoglycans)
- Pharmaceuticals (detection, development, characterisation).
G) EXPERIENCE SUMMARY
- Twenty three years research experience covering carbohydrate/protein biochemistry, molecular interactions, assay development, macromolecule purification/analysis, cell signalling and microbial adhesion.
- First-hand experience of commercial science, including intellectual property exploitation and contract research as cofounder/director of university spin-out drug discovery company: IntelliHep Ltd.
- Member of UK Glycoarrays Consortium and IBCarb & CBMNet Networks in Industrial Biotechnology, Biochemical Society Local Ambassador, Fellow Higher Education Academy.
2012, Liverpool John Moores University, United Kingdom, Postgraduate Certificate for Learning and Teaching in Higher Education (High distinction)
2001, University of Birmingham, United Kingdom, PhD (Biochemistry)
1996, University of Oxford, United Kingdom, MBiochem with honours (Biochemistry)
Lecturer/Senior Lecturer, Pharmacy & Biomolecular Science, Liverpool John Moores University, 2008 - present
Honarary Fellow, Institute of Integrative Biology, University of Liverpool, 2008 - present
Postdoctoral Research Fellow, United Kingdom, University of Liverpool, 2004 - 2008
Postdoctoral Research Fellow, United Kingdom, University of Birmingham, 2001 - 2003
Postgraduate Research Assistant, United Kingdom, University of Birmingham, 2001 - 2001
Postgraduate Research Assistant, United Kingdom, University of Oxford, 1996 - 1997
Richardson A, Powell A, Sexton D, Parsons J, Reynolds N, Ross K. 2020. MicroRNA-184 Is Induced By Store-Operated Calcium Entry And Regulates Early Keratinocyte Differentiation Journal of Cellular Physiology, DOI Author Url Publisher Url Public Url
Maciej-Hulme ML, Leprince ACN, Lavin A, Guimond SE, Turnbull JE, Pelletier J, Yates EA, Powell AK, Skidmore MA. 2020. High sensitivity (zeptomole) detection of BODIPY heparan sulfate (HS) disaccharides by ion-paired RP-HPLC and LIF detection enables analysis of HS from mosquito midguts. bioRxiv, DOI Publisher Url
Adeoye RI, Osalaye DS, Ralebitso-Senior TK, Boddis A, Reid AJ, Fatokun AA, Powell AK, Malomo SO, Olorunniji FJ. 2019. Catalytic Activities of Multimeric G-Quadruplex DNAzymes Catalysts, 9 :613-613 DOI Author Url Public Url
Lima M, Cavalheiro RP, Viana G, Meneghetti M, Rudd TR, Skidmore MA, Powell AK, Yates EA. 2016. 19F labelled glycosaminoglycan probes for solution NMR and non-linear (CARS) microscopy. Glycoconjugate Journal, 34 :405-410 DOI Author Url Public Url
Ahmed YA, Yates EA, Moss DJ, Loeven MA, Hussain S-A, Hohenester E, Turnbull JE, Powell AK. 2016. Panels of chemically-modified heparin polysaccharides and natural heparan sulfate saccharides both exhibit differences in binding to Slit and Robo, as well as variation between protein binding and cellular activity. Molecular Biosystems, DOI Author Url Public Url
Meneghetti MC, Hughes AJ, Rudd TR, Nader HB, Powell AK, Yates EA, Lima MA. 2015. Heparan sulfate and heparin interactions with proteins. Journal of the Royal Society Interface, 12 DOI Author Url Public Url
Solari V, Rudd TR, Guimond SE, Powell AK, Turnbull JE, Yates EA. 2015. Heparan sulfate phage display antibodies recognise epitopes defined by a combination of sugar sequence and cation binding. Organic and Biomolecular Chemistry, 13 :6066-6072 DOI Public Url
Otto DME, Campanero-Rhodes MA, Karamanska R, Powell AK, Bovin N, Turnbull JE, Field RA, Blackburn J, Feizi T, Crocker PR. 2011. An expression system for screening of proteins for glycan and protein interactions. Anal Biochem, 411 :261-270 DOI Author Url
Tissot B, Ceroni A, Powell AK, Morris HR, Yates EA, Turnbull JE, Gallagher JT, Dell A, Haslam SM. 2008. Software tool for the structural determination of glycosaminoglycans by mass spectrometry. Anal Chem, 80 :9204-9212 DOI Author Url
Zhi Z-L, Laurent N, Powell AK, Karamanska R, Fais M, Voglmeir J, Wright A, Blackburn JM, Crocker PR, Russell DA, Flitsch S, Field RA, Turnbull JE. 2008. A versatile gold surface approach for fabrication and interrogation of glycoarrays. Chembiochem, 9 :1568-1575 DOI Author Url
Hussain S-A, Piper M, Fukuhara N, Strochlic L, Cho G, Howitt JA, Ahmed Y, Powell AK, Turnbull JE, Holt CE, Hohenester E. 2006. A molecular mechanism for the heparan sulfate dependence of slit-robo signaling. J Biol Chem, 281 :39693-39698 DOI Author Url
Zhi Z-L, Powell AK, Turnbull JE. 2006. Fabrication of carbohydrate microarrays on gold surfaces: direct attachment of nonderivatized oligosaccharides to hydrazide-modified self-assembled monolayers. Anal Chem, 78 :4786-4793 DOI Author Url
Powell AK, Fernig DG, Turnbull JE. 2002. Fibroblast growth factor receptors 1 and 2 interact differently with heparin/heparan sulfate. Implications for dynamic assembly of a ternary signaling complex. J Biol Chem, 277 :28554-28563 DOI Author Url
Rudd TR, Skidmore MA, Guerrini M, Hricovini M, Powell AK, Siligardi G, Yates EA. The conformation and structure of GAGs: Recent progress and perspectives. Curr Opin Str Biol, 20 :567-574 DOI Author Url
Tissot B, Gasiunas N, Powell AK, Ahmed Y, Zhi Z-L, Haslam SM, Morris HR, Turnbull JE, Gallagher JT, Dell A. Towards GAG glycomics: analysis of highly sulfated heparins by MALDI-TOF mass spectrometry. Glycobiology, 17 :972-982 DOI Author Url
Powell AK, Yates EA, Fernig DG, Turnbull JE. Interactions of heparin/heparan sulfate with proteins: appraisal of structural factors and experimental approaches. Glycobiology, 14 :17R-30R DOI Author Url
Yates EA, Jones MO, Clarke CE, Powell AK, Johnson SR, Porch A, Edwards PP, Turnbull JE. Microwave-enhanced reaction of carbohydrates with amino-derivatised labels and glass surfaces. J. Mater. Chem., 13 :2061-2063 DOI
Guile GR, Harvey DJ, O’Donnell N, Powell AK, Hunter AP, Zamze S, Fernandes DL, Dwek RA, Wing DR. Identification of highly fucosylated N-linked oligosaccharides from the human parotid gland. Eur. J. Biochem., 258 :623-656 DOI Author Url
Powell AK, Harvey DJ. Stabilisation of sialic acids in N-linked oligosaccharides and gangliosides for analysis by positive ion matrix-assisted laser desorption/ionization mass spectrometry. Rapid. Commun. Mass Spectrom., 10 :1027-1032 DOI Author Url
Richardson A, Sexton D, Powell AK, Ross K. 2017. A store-operated calcium entry-dependent regulatory loop between nuclear factor of activated T-cells 1 and miR-184 in keratinocytes Non-Coding RNA: Recent Insights into the Mechanism of Action, Biochemical Society Conference, Edinburgh, UK.
Richardson A, Sexton D, Powell AK, Ross K. 2016. Argonaute Expression in Psoriasis and Epidermal Keratinocytes. Inflammatory diseases - mechanisms and clinical perspectives, Maynooth University, Ireland. A Biochemical Society Focused Meeting.
Ahmed YA, Yates EA, Hohenester E, Powell AK, Turnbull JE. 2014. Selective effects of chemically modified heparins and heparan sulfate oligosaccharides on Slit & Robo complex formation and biological activity. Proteoglycans, Gordon Research Conference, Proctor Academy, Andover, NH, USA
Ahmed YA, Loeven MA, Hussain S, Yates EA, Moss DJ, Hohenester E, Turnbull JE, Powell AK. 2014. Selective effects of chemically modified heparins on Slit & Robo complex formation and biological activity 27th International Carbohydrate Symposium (ICS27), Bangalore, India.
Ahmed YA, Atrih AM, Guimond SE, Powell AK, Turnbull JE. 2012. Sequence-specific HS modulation of FGF signalling revealed by an ES-MS sequencing strategy Gordon Research Conference on Fibroblast Growth Factors in Development & Disease, Les Diablerets, Switzerland
Powell A, Zhi Z-L , Ahmed Y, Karamanska R, Fais M, Otto DME, Crocker RP, Yates EA, Field RA, Turnbull JE. 2010. Heparan sulfate saccharide arrays – platform development, library screening and data interpretation. Structural glycobiology and human health, Biochemical Society conference
Ahmed YA, Powell AK, Yates EA, Moss D, Hohenester E, Turnbull JE. 2010. Involvement of Heparan Sulfate in Slit-Robo interactions: Evidence that Specific Sulfate Groups are Essential Structural Glycobiology and human health, Royal Holloway, University of London, UK
Powell A, Zhi Z-L, Ahmed Y, Karamanska R, Fais M, Otto DME, Rudd TR, Blackburn JM, Russell DA, Crocker PR. 2009. Development and evaluation of heparan sulfate glycoarrays for protein interaction screening 67th Harden conference (Biochemical Society) - Decoding the biology of heparan sulfate proteoglycans, Robinson College, Cambridge,
Ahmed YA, Powell AK, Yates EA, Moss D, Hohenester E, Turnbull JE. 2009. Involvement of Heparan Sulfate in Slit-Robo interactions: Evidence that Specific Sulfate Groups are Essential The 67th Harden conference: Decoding the biology of heparan sulfate proteoglycans, Robinson College, Cambridge, UK
Powell AK, Ahmed Y, Zhi Z-L, Rudd TR, Nichols R, Fernig DG, Yates EA, Turnbull JE. 2007. Saccharide microarrays for the investigation of heparan-sulfate protein interactions 14th European carbohydrate symposium
Ahmed YA, Powell AK, Guimond S, Atrih A, Turnbull JE. 2007. Investigating the specificity of heparin/ heparan sulfate activity and protein interactions using saccharide libraries FEBS Advanced Lecture Course, Matrix Pathobiology, Signaling and Molecular Targets. Patras, Greece
Coyne V, Ross K, Shriver Z, Wills B, Powell AK. Applying an HPLC structural glycomics strategy to investigate triggers of changes in HS structure Carbohydrate analysis and glycomics: where next? Biochemical Society/Royal Society of Chemistry workshop
Joshi KH, Al-Manasra M, Mason A, Korostynska O, Powell A, Ortoneda Pedrola M, Al-Shamma'a A. 2017. Detection of Heparin Level in Blood using Electromagnetic Wave Spectroscopy Developments in eSystems Engineering (DeSE), 2016 9th International Conference on :329-334 DOI Author Url Publisher Url
Richardson A, Sexton D, Powell AK, Ross K. 2017. Defining the Role of Argonautes in Human Epidermal Keratinocytes British Society for Investigative Dermatology Annual Meeting 176 :e71-e71
Lavin A, Powell AK. 2014. 2013 Biochemical Society Summer Vacation Studentship Report Heparan sulfate disaccharide analysis using fluorescence labelling and high performance liquid chromatography. http://www.scribd.com/doc/201219149/Summer-Students-Reports-2013
Coyne V, Ross KO, Powell AK. 2011 Biochemical Society Summer Vacation Studentship Report Examining the effects of cytokines on heparan sulfate expression in a HaCaT cell line :193-196
Research Grants Awarded:
LJMU Joint Faculty of Science/Faculty of Health PhD Studentships, Identification of blood biomarkers for antenatal diagnosis of a common congenital heart defect, Tetralogy of Fallot., Iain Dykes, Andrew Powell, Robyn Lotto, Atillio Lotto, Grant value (£): ~$62,000, Duration of research project: 3 years. 2020
LJMU REF-upskilling, Development of split G-Quadruplex DNAzymes for construction of gated biosensors, Femi Olorunniji, Andrew Powell, Amos Fatokun, Theresia Rabelitso-Senior, Amanda Boddis, Amanda Reid, Grant value (£): 15000, Duration of research project: 1 year. 2018
Rosetrees Trust, Novel carbohydrate antibiotics for the era of antibiotic resistance, Andrew Powell, Grant value (£): 19,000, Duration of research project: 1.5 year. 2018
IBCarb BBSRC Network in Industrial Biotechnology, Enabling heparin-based industrial biotechnology to counteract antibiotic resistance., Grant value (£): 19,728, Duration of research project: 9 months. 2018
Medical Research Council Industry Engagement Fund 2017-18, Towards novel heparin-based antibiotics for bacterial infection, Grant value (£): 5,000, Duration of research project: 5 months. 2018
HEFCE, Enabling discovery of novel heparin-based therapeutics, Grant value (£): 9951, Duration of research project: 3 months. 2017
HEFCE-QR, Hepsense: Next generation sensors of heparin-type carbohydrates - open heart surgery & beyond., Grant value (£): 63,000, Duration of research project: 1.5 years (truncated). 2017
BBSRC/EPSRC, Advanced glycosaminoglycan sequencing (Collaborator), Dr Mark Skidmore, Grant value (£): £330,445, Duration of research project: 3 years. 2014
Biochemical Society Eric Reid Fund for Methodology, Quantification of heparin/heparan sulfate carbohydrates, Grant value (£): 1,880, Duration of research project: 3 months. 2014
Biochemical Society Summer Vacation Studentship, Developing higher sensitivity High Performance Liquid Chromatography disaccharide composition analysis of heparan sulfate carbohydrates, Grant value (£): 1,400, Duration of research project: 2 months. 2013
Biochemical Society Summer Vacation Studentship, Regulation of keratinocyte heparan sulfate expression., Grant value (£): 1,600, Duration of research project: 2 months. 2011
HEFCE-QR, Characterisation of the regulation of epithelial FGF systems by heparan sulphate using the HaCaT keratinocyte cell line in different states as a model., Grant value (£): ~57,000, Duration of research project: 1.5 years (truncated). 2010
EPSRC, ‘Exploitation of Glycoarrays-Translation to End-use, Haslam SM, Dell A, Feizi T, Crocker PR, Powell AK, JE Turnbull, Russell DA, RA Field, S Flitsch:, Grant value (£): 1,030,438, Duration of research project: 2.5 years. 2009
North West Development Agency National Biomanufacturing Centre Access Fund, Heparin-based saccharide production procedure development Part 2, Powell AK (IntelliHep Ltd), Grant value (£): 68,000, Duration of research project: 1 year. 2008
North West Development Agency National Biomanufacturing Centre Access Fund, Heparin-based saccharide production procedure development Part 1, Powell AK (IntelliHep Ltd), Grant value (£): 68,000, Duration of research project: 1 year. 2007
BBSRC, Exploitation of heparan sulfate saccharide microarrays, Powell AK, Fernig DG, Yates EA and Turnbull JE, Grant value (£): 274,000, Duration of research project: 3 years. 2004
BBSRC, Development of heparan sulfate saccharide microarrays, Powell AK and Turnbull, JE, Grant value (£): 99,000, Duration of research project: 2 years. 2001
Other invited event:
Glycobiotechnology, Manchester, UK, Investigating skin-antibiotic activities. 2018
IBCarb Glycan Microarrays 2.0, Manchester Institute of Biotechnology, Manchester, Oral presentation - Heparan sulfate interaction studies. 2014
Gordon Research Seminar: Fibroblast Growth Factors in Development & Disease, Les Diablerets Conference Center, Switzerland, Discussion Leader (FGF interactions and signalling). Panel member (Becoming an independent researcher).. 2012
University of Keele Seminar, School of Life Sciences, The Institute for Science & Technology in Medicine, Keele University, Oral presentation - Understanding heparan sulfate carbohydrates: Viral infection and glycoarrays.. 2012
Biochemical Society/Royal Society of Chemistry workshop: Carbohydrate analysis and glycomics: where next?, London, Oral presentation - Applying an HPLC structural glycomics strategy to investigate triggers of changes in heparan sulfate.. 2012
67th Harden conference (Biochemical Society) - Decoding the biology of heparan sulfate proteoglycans, Robinson College, Cambridge, UK, Oral presentation - Development and evaluation of heparan sulfate glycoarrays for protein interaction screening. 2009
Royal Society Glycomics conference, London, Oral presentation - Slide-based fluorescence glycoarrays. 2008
14th European carbohydrate symposium, Lubeck, Germany, Oral presentation - Saccharide microarrays for the investigation of heparan-sulfate protein interactions. 2007
Royal Society Glycomics conference, Liverpool, Oral presentation - The challenge of heparan sulfate microarrays. 2006
Postgraduate Certificate in Higher Education. 2014
Fellow, Higher Education Academy. 2012
Honarary Fellow, Institute of Integrative Biology, University of Liverpool. 2008
External PGR Supervision - completed students:
University of Liverpool, PhD. 2011
Membership of professional bodies:
Local Ambassador, Biochemical Society. 2010
Emerging Technology Project of the Year (runner up), BioNoW. 2008
BioNow North West Young Biotechnologist of the Year., BioNow. 2005
IntelliHep Ltd, Liverpool, UK, Founder & Director. 2002
http://www.bbsrc.ac.uk/news/impact/complex-sugars/, BBSRC impact evidence reports: Carbohydrate based drugs - IntelliHep Ltd..