Liverpool Skylilne

Pharmacy and Biomolecular Sciences

Dr Andrew Powell

Dr Andrew Powell

Telephone: 0151 231 2487

Biography

I develop and exploit analytical biochemistry methods to both detect/quantify biological molecules and to characterise their molecular interactions to understand structure-function relationships.

I have mostly focussed on proteins and carbohydrates. An example of the latter are long sulfated carbohydrates from the heparin/heparan sulphate family. These are existing pharmaceuticals used to prevent blood clotting ($8 billion market). They are also known bind/regulate 100-1000s of extracellular proteins linked to inflammation, infection, cancer, neurodegeneration and regenerative biology hence are a rich resource for drug discovery.

I currently use mainly chromatography, bioconjugation chemistry, plate readers, thermostability/differential scanning fluorimetry, flow cytometry and cellular assays. I collaborate with cell biologists, biophysicists, synthetic chemists and engineers. I am also interested in the manufacture, formulation/delivery and monitoring of these molecules as current/future pharmaceuticals.

Equipment available includes multiple BMG and Tecan plate readers (absorbance, fluorescence intensity, fluorescence polarisation), BD flow cytometry, Qiagen Q-PCR, HPLCs, Bruker NMR and cell culture.

Funding is obtained from the research councils and biochemical society.

INTERESTS SUMMARY
- Proteins (e.g. growth factors, cytokines, chemokines, proteases)
- Carbohydrates (e.g. heparin/heparan sulfate/glycosaminoglycans)
- Method Development ( immunoassays, plate reader assays, bioassays, chromatography, optical biosensors, microarray assays).
- Analytical Biochemistry (molecular interactions, structure-function relationships, macromolecule purification & analysis).
- Cell Biology (cell signaling, microbial adhesion, homeostasis & immunity of epithelia, blood coagulation).
- Pharmaceuticals (detection, development, characterisation and formulation/delivery).

EXPERIENCE
- Twenty years research experience covering carbohydrate/protein biochemistry, molecular interactions, assay development, macromolecule purification/analysis, cell signalling and microbial adhesion.
- First-hand experience of commercial science, including intellectual property exploitation and contract research as cofounder/director of university spin-out drug discovery company: IntelliHep Ltd.
- Member of UK Glycoarrays Consortium and IBCarb & CBMNet Networks in Industrial Biotechnology, Biochemical Society Local Ambassador, Fellow Higher Education Academy.
- Supervised ~ 55 honours project students, 5 summer students/interns, ~10 masters/PhD students, 2 postdoctoral associates.
- Lecturing of protein and carbohydrate structure, purification, analysis and interactions at undergraduate and masters level with a focus on technical approaches (numerous nominations/shortlistings for university teaching, personal tutor, project supervisor and employability awards).

EMPLOYMENT
08 – to date. Senior Lecturer in Biochemistry, Liverpool John Moores University, UK.
08 – to date. Honarary Fellow, University of Liverpool, UK.
02 – to date. CoFounder & Director, IntelliHep Ltd, UK.
07 – 08. Postdoctoral Research Fellow (EPSRC), University of Liverpool, UK.
04 – 07. Postdoctoral Research Fellow (BBSRC), University of Liverpool, UK.
01 – 03. Postdoctoral Research Fellow (BBSRC), University of Birmingham, UK.
97 – 01. PhD student (MRC), University of Birmingham, UK.
96 – 97. Postgraduate Research Assistant, University of Oxford, UK.

Degrees

2012, Liverpool John Moores University, United Kingdom, Postgraduate Certificate for Learning and Teaching in Higher Education (High distinction)
2001, University of Birmingham, United Kingdom, PhD (Biochemistry)
1996, University of Oxford, United Kingdom, MBiochem with honours (Biochemistry)

Academic appointments

Lecturer/Senior Lecturer, Pharmacy & Biomolecular Science, Liverpool John Moores University, 2008 - present
Honarary Fellow, Institute of Integrative Biology, University of Liverpool, 2008 - present
Postdoctoral Research Fellow, School of Biological Sciences, University of Liverpool, 2004 - 2008
Postdoctoral Research Fellow, School of Bioscience, University of Birmingham, 2001 - 2003
Postgraduate Research Assistant, School of Bioscience, University of Birmingham, 2001 - 2001
MRC PhD student, School of Bioscience, University of Birmingham, 1997 - 2001
Postgraduate Research Assistant, Glycobiology Institute, University of Oxford, 1996 - 1997

Postgraduate training

Postdoctoral Research Fellow, United Kingdom, University of Liverpool, 2004 - 2008
Postdoctoral Research Fellow, United Kingdom, University of Birmingham, 2001 - 2003
Postgraduate Research Assistant, United Kingdom, University of Birmingham, 2001 - 2001

Publications

Highlighted publications

Ahmed YA, Yates EA, Moss DJ, Loeven MA, Hussain S-A, Hohenester E, Turnbull JE, Powell AK. 2016. Panels of chemically-modified heparin polysaccharides and natural heparan sulfate saccharides both exhibit differences in binding to Slit and Robo, as well as variation between protein binding and cellular activity. Molecular Biosystems, >DOI

Lima M, Cavalheiro RP, Viana G, Meneghetti M, Rudd TR, Skidmore MA, Powell AK, Yates EA. 2016. 19F labelled glycosaminoglycan probes for solution NMR and non-linear (CARS) microscopy. Glycoconjugate Journal, >DOI

Powell AK, Ahmed YA, Yates EA, Turnbull JE. 2010. Generating heparan sulfate saccharide libraries for glycomics applications. Nat Protoc, 5 :821-833 >DOI >Link

Powell AK, Zhi Z-L, Turnbull JE. 2009. Saccharide microarrays for high-throughput interrogation of glycan-protein binding interactions. Methods Mol Biol, 534 :313-329 >DOI >Link

Tissot B, Gasiunas N, Powell AK, Ahmed Y, Zhi Z-L, Haslam SM, Morris HR, Turnbull JE, Gallagher JT, Dell A. 2007. Towards GAG glycomics: analysis of highly sulfated heparins by MALDI-TOF mass spectrometry. Glycobiology, 17 :972-982 >DOI >Link

Powell AK, Yates EA, Fernig DG, Turnbull JE. 2004. Interactions of heparin/heparan sulfate with proteins: appraisal of structural factors and experimental approaches. Glycobiology, 14 :17R-30R >DOI >Link

Westblade LF, Ilag LL, Powell AK, Kolb A, Robinson CV, Busby SJW. 2004. Studies of the Escherichia coli Rsd-sigma70 complex. J Mol Biol, 335 :685-692 >Link

Powell AK, Fernig DG, Turnbull JE. 2002. Fibroblast growth factor receptors 1 and 2 interact differently with heparin/heparan sulfate. Implications for dynamic assembly of a ternary signaling complex. J Biol Chem, 277 :28554-28563 >DOI >Link

Poster

Richardson A, Sexton D, Powell AK, Ross K. 2017. A store-operated calcium entry-dependent regulatory loop between nuclear factor of activated T-cells 1 and miR-184 in keratinocytes Non-Coding RNA: Recent Insights into the Mechanism of Action, Biochemical Society Conference, Edinburgh, UK

Richardson A, Sexton D, Powell AK, Ross K. 2017. Defining the Role of Argonautes in Human Epidermal Keratinocytes British Society for Investigative Dermatology Annual Meeting, Manchester, UK

Richardson A, Sexton D, Powell AK, Ross K. 2016. Argonaute Expression in Psoriasis and Epidermal Keratinocytes. Inflammatory diseases - mechanisms and clinical perspectives, Maynooth University, Ireland. A Biochemical Society Focused Meeting.

Ahmed YA, Loeven MA, Hussain S, Yates EA, Moss DJ, Hohenester E, Turnbull JE, Powell AK. 2014. Selective effects of chemically modified heparins on Slit & Robo complex formation and biological activity 27th International Carbohydrate Symposium (ICS27), Bangalore, India.

Ahmed YA, Yates EA, Hohenester E, Powell AK, Turnbull JE. 2014. Selective effects of chemically modified heparins and heparan sulfate oligosaccharides on Slit & Robo complex formation and biological activity. Proteoglycans, Gordon Research Conference, Proctor Academy, Andover, NH, USA

Ahmed YA, Atrih AM, Guimond SE, Powell AK, Turnbull JE. 2012. Sequence-specific HS modulation of FGF signalling revealed by an ES-MS sequencing strategy Gordon Research Conference on Fibroblast Growth Factors in Development & Disease, Les Diablerets, Switzerland

Coyne V, Ross K, Shriver Z, Wills B, Powell AK. 2012. Applying an HPLC structural glycomics strategy to investigate triggers of changes in HS structure Carbohydrate analysis and glycomics: where next? Biochemical Society/Royal Society of Chemistry workshop

Ahmed YA, Powell AK, Yates EA, Moss D, Hohenester E, Turnbull JE. 2010. Involvement of Heparan Sulfate in Slit-Robo interactions: Evidence that Specific Sulfate Groups are Essential Structural Glycobiology and human health, Royal Holloway, University of London, UK

Powell A, Zhi Z-L , Ahmed Y, Karamanska R, Fais M, Otto DME, Crocker RP, Yates EA, Field RA, Turnbull JE. 2010. Heparan sulfate saccharide arrays – platform development, library screening and data interpretation. Structural glycobiology and human health, Biochemical Society conference

Ahmed YA, Powell AK, Yates EA, Moss D, Hohenester E, Turnbull JE. 2009. Involvement of Heparan Sulfate in Slit-Robo interactions: Evidence that Specific Sulfate Groups are Essential The 67th Harden conference: Decoding the biology of heparan sulfate proteoglycans, Robinson College, Cambridge, UK

Powell A, Zhi Z-L, Ahmed Y, Karamanska R, Fais M, Otto DME, Rudd TR, Blackburn JM, Russell DA, Crocker PR. 2009. Development and evaluation of heparan sulfate glycoarrays for protein interaction screening 67th Harden conference (Biochemical Society) - Decoding the biology of heparan sulfate proteoglycans, Robinson College, Cambridge,

Ahmed YA, Powell AK, Guimond S, Atrih A, Turnbull JE. 2007. Investigating the specificity of heparin/ heparan sulfate activity and protein interactions using saccharide libraries FEBS Advanced Lecture Course, Matrix Pathobiology, Signaling and Molecular Targets. Patras, Greece

Powell AK, Ahmed Y, Zhi Z-L, Rudd TR, Nichols R, Fernig DG, Yates EA, Turnbull JE. 2007. Saccharide microarrays for the investigation of heparan-sulfate protein interactions 14th European carbohydrate symposium

Journal article

Ahmed YA, Yates EA, Moss DJ, Loeven MA, Hussain S-A, Hohenester E, Turnbull JE, Powell AK. 2016. Panels of chemically-modified heparin polysaccharides and natural heparan sulfate saccharides both exhibit differences in binding to Slit and Robo, as well as variation between protein binding and cellular activity. Molecular Biosystems, >DOI

Lima M, Cavalheiro RP, Viana G, Meneghetti M, Rudd TR, Skidmore MA, Powell AK, Yates EA. 2016. 19F labelled glycosaminoglycan probes for solution NMR and non-linear (CARS) microscopy. Glycoconjugate Journal, >DOI

Meneghetti MC, Hughes AJ, Rudd TR, Nader HB, Powell AK, Yates EA, Lima MA. 2015. Heparan sulfate and heparin interactions with proteins. Journal of the Royal Society Interface, 12 >DOI

Solari V, Rudd TR, Guimond SE, Powell AK, Turnbull JE, Yates EA. 2015. Heparan sulfate phage display antibodies recognise epitopes defined by a combination of sugar sequence and cation binding. Organic and Biomolecular Chemistry, 13 :6066-6072 >DOI

Puvirajesinghe TM, Ahmed YA, Powell AK, Fernig DG, Guimond SE, Turnbull JE. 2012. Array-based Functional Screening of Heparin Glycans. Chemistry & Biology, 19 :553-558

Otto DME, Campanero-Rhodes MA, Karamanska R, Powell AK, Bovin N, Turnbull JE, Field RA, Blackburn J, Feizi T, Crocker PR. 2011. An expression system for screening of proteins for glycan and protein interactions. Anal Biochem, 411 :261-270 >DOI >Link

Powell AK, Ahmed YA, Yates EA, Turnbull JE. 2010. Generating heparan sulfate saccharide libraries for glycomics applications. Nat Protoc, 5 :821-833 >DOI >Link

Rudd TR, Skidmore MA, Guerrini M, Hricovini M, Powell AK, Siligardi G, Yates EA. 2010. The conformation and structure of GAGs: Recent progress and perspectives. Curr Opin Str Biol, 20 :567-574

Powell AK, Zhi Z-L, Turnbull JE. 2009. Saccharide microarrays for high-throughput interrogation of glycan-protein binding interactions. Methods Mol Biol, 534 :313-329 >DOI >Link

Zhi Z-L, Laurent N, Powell AK, Karamanska R, Fais M, Voglmeir J, Wright A, Blackburn JM, Crocker PR, Russell DA, Flitsch S, Field RA, Turnbull JE. 2008. A versatile gold surface approach for fabrication and interrogation of glycoarrays. Chembiochem, 9 :1568-1575 >DOI >Link

Tissot B, Ceroni A, Powell AK, Morris HR, Yates EA, Turnbull JE, Gallagher JT, Dell A, Haslam SM. 2008. Software tool for the structural determination of glycosaminoglycans by mass spectrometry. Anal Chem, 80 :9204-9212 >DOI >Link

Tissot B, Gasiunas N, Powell AK, Ahmed Y, Zhi Z-L, Haslam SM, Morris HR, Turnbull JE, Gallagher JT, Dell A. 2007. Towards GAG glycomics: analysis of highly sulfated heparins by MALDI-TOF mass spectrometry. Glycobiology, 17 :972-982 >DOI >Link

Hussain S-A, Piper M, Fukuhara N, Strochlic L, Cho G, Howitt JA, Ahmed Y, Powell AK, Turnbull JE, Holt CE, Hohenester E. 2006. A molecular mechanism for the heparan sulfate dependence of slit-robo signaling. J Biol Chem, 281 :39693-39698 >DOI >Link

Zhi Z-L, Powell AK, Turnbull JE. 2006. Fabrication of carbohydrate microarrays on gold surfaces: direct attachment of nonderivatized oligosaccharides to hydrazide-modified self-assembled monolayers. Anal Chem, 78 :4786-4793 >DOI >Link

Powell AK, Yates EA, Fernig DG, Turnbull JE. 2004. Interactions of heparin/heparan sulfate with proteins: appraisal of structural factors and experimental approaches. Glycobiology, 14 :17R-30R >DOI >Link

Westblade LF, Ilag LL, Powell AK, Kolb A, Robinson CV, Busby SJW. 2004. Studies of the Escherichia coli Rsd-sigma70 complex. J Mol Biol, 335 :685-692 >Link

Yates EA, Jones MO, Clarke CE, Powell AK, Johnson SR, Porch A, Edwards PP, Turnbull JE. 2003. Microwave-enhanced reaction of carbohydrates with amino-derivatised labels and glass surfaces. J. Mater. Chem., 13 :2061-2063

Powell AK, Fernig DG, Turnbull JE. 2002. Fibroblast growth factor receptors 1 and 2 interact differently with heparin/heparan sulfate. Implications for dynamic assembly of a ternary signaling complex. J Biol Chem, 277 :28554-28563 >DOI >Link

Turnbull J, Powell A, Guimond S. 2001. Heparan sulfate: decoding a dynamic multifunctional cell regulator. Trends Cell Biol, 11 :75-82 >Link

Guile GR, Harvey DJ, O’Donnell N, Powell AK, Hunter AP, Zamze S, Fernandes DL, Dwek RA, Wing DR. 1998. Identification of highly fucosylated N-linked oligosaccharides from the human parotid gland. Eur. J. Biochem., 258 :623-656

Powell AK, Harvey DJ. 1996. Stabilisation of sialic acids in N-linked oligosaccharides and gangliosides for analysis by positive ion matrix-assisted laser desorption/ionization mass spectrometry. Rapid. Commun. Mass Spectrom., 10 :1027-1032

Report

Lavin A, Powell AK. 2014. 2013 Biochemical Society Summer Vacation Studentship Report Heparan sulfate disaccharide analysis using fluorescence labelling and high performance liquid chromatography. http://www.scribd.com/doc/201219149/Summer-Students-Reports-2013

Coyne V, Ross KO, Powell AK. 2011. 2011 Biochemical Society Summer Vacation Studentship Report Examining the effects of cytokines on heparan sulfate expression in a HaCaT cell line :193-196

Engagement & Impact

Highlighted activities

Fellowships:

Fellowship title: Fellow, Organisation: Higher Education Academy

Fellowship title: Honarary Fellow, Organisation: Institute of Integrative Biology, University of Liverpool

Industrial connections:

Company: IntelliHep Ltd, Liverpool, UK, Position or project: Founder & Director

Membership of professional bodies:

Local Ambassador, Biochemical Society

Other invited event:

Title of event: IBCarb Glycan Microarrays 2.0, Location: Manchester Institute of Biotechnology, Manchester, Description: Oral presentation - Heparan sulfate interaction studies

Professional activities

Award:

Award title: Emerging Technology Project of the Year (runner up), Awarding body: BioNoW

Award title: BioNow North West Young Biotechnologist of the Year., Awarding body: BioNow

External collaboration:

Institution: Diamond Light Source Ltd, Collaborator: Dr Ashley Hughes (Marie Sklodowska-Curie Fellow), B23 Circular Dichroism Beamline.

Institution: Institute of Integrative Biology, University of Liverpool, UK, Collaborator: Dr Malcolm Horsburgh

Institution: Department of Life Sciences, Imperial College London, UK, Collaborator: Professor Erhard Hohenester

Institution: Disciplina de Biologia Molecular, Departamento de Bioquímica, Universidade Federal de São Paulo, São Paulo, Brazil., Collaborator: Dr Lima MA & Professor Nader HB

Institution: Institute for Science & Technology in Medicine, University of Keele, UK, Collaborator: Dr Mark Skidmore

Institution: Institute of Integrative Biology, University of Liverpool, UK, Collaborator: Professor Jeremy Turnbull, Dr Ed Yates & Dr S Guimond

External PGR Supervision - completed students:

Name of University: University of Liverpool, Award gained by the student: PhD

Fellowships:

Fellowship title: Fellow, Organisation: Higher Education Academy

Fellowship title: Honarary Fellow, Organisation: Institute of Integrative Biology, University of Liverpool

Industrial connections:

Company: IntelliHep Ltd, Liverpool, UK, Position or project: Founder & Director

Media Coverage:

Links #1: http://www.bbsrc.ac.uk/news/impact/complex-sugars/, Media coverage: BBSRC impact evidence reports: Carbohydrate based drugs - IntelliHep Ltd.

Membership of professional bodies:

Local Ambassador, Biochemical Society

Other invited event:

Title of event: Royal Society Glycomics conference, Location: Liverpool, Description: Oral presentation - The challenge of heparan sulfate microarrays

Title of event: 14th European carbohydrate symposium, Location: Lubeck, Germany, Description: Oral presentation - Saccharide microarrays for the investigation of heparan-sulfate protein interactions

Title of event: Royal Society Glycomics conference, Location: London, Description: Oral presentation - Slide-based fluorescence glycoarrays

Title of event: 67th Harden conference (Biochemical Society) - Decoding the biology of heparan sulfate proteoglycans, Location: Robinson College, Cambridge, UK, Description: Oral presentation - Development and evaluation of heparan sulfate glycoarrays for protein interaction screening

Title of event: IBCarb Glycan Microarrays 2.0, Location: Manchester Institute of Biotechnology, Manchester, Description: Oral presentation - Heparan sulfate interaction studies

Title of event: Biochemical Society/Royal Society of Chemistry workshop: Carbohydrate analysis and glycomics: where next?, Location: London, Description: Oral presentation - Applying an HPLC structural glycomics strategy to investigate triggers of changes in heparan sulfate.

Title of event: University of Keele Seminar, Location: School of Life Sciences, The Institute for Science & Technology in Medicine, Keele University, Description: Oral presentation - Understanding heparan sulfate carbohydrates: Viral infection and glycoarrays.

Title of event: Gordon Research Seminar: Fibroblast Growth Factors in Development & Disease, Location: Les Diablerets Conference Center, Switzerland, Description: Discussion Leader (FGF interactions and signalling). Panel member (Becoming an independent researcher).

Research Grants Awarded:

HEFCE-QR, Hepsense: Next generation sensors of heparin-type carbohydrates - open heart surgery & beyond., Grant value (£): 63,000

HEFCE-QR, Characterisation of the regulation of epithelial FGF systems by heparan sulphate using the HaCaT keratinocyte cell line in different states as a model., Grant value (£): ~57,000

BBSRC/EPSRC, Advanced glycosaminoglycan sequencing (Collaborator), Dr Mark Skidmore, Grant value (£): £330,445

BBSRC, Exploitation of heparan sulfate saccharide microarrays, Powell AK, Fernig DG, Yates EA and Turnbull JE, Grant value (£): 274,000

BBSRC, Development of heparan sulfate saccharide microarrays, Powell AK and Turnbull, JE, Grant value (£): 99,000

North West Development Agency, National Biomanufacturing Centre Access Fund, Powell AK (IntelliHep Ltd), Grant value (£): 136,000

Chamada MCTI/CNPQ/FAPS No 16/2014. programma INCT, A National Institute of Integrative Glycobiology: At the Crossroads of health and Disease' (Collaborator)., Professor HB Nader, Dr MA Lima, Departamento de Bioquímica, Universidade Federal de São Paulo (UNIFESP), Brazil, Grant value (£): R$ 9,890,307 (Brazil)

Biochemical Society, Quantification of heparin/heparan sulfate carbohydrates, Grant value (£): 1880

Biochemical Society Summer Vacation Studentship, Developing higher sensitivity Reverse Phase-High Performance Liquid Chromatography disaccharide composition analysis of heparan sulfate carbohydrates using BODIPY fluorescence detection, Grant value (£): 1400

EPSRC, ‘Exploitation of Glycoarrays-Translation to End-use, Haslam SM, Dell A, Feizi T, Crocker PR, Powell AK, JE Turnbull, Russell DA, RA Field, S Flitsch:, Grant value (£): 1,030,438

Biochemical Society Summer Vacation Studentship, Regulation of keratinocyte heparan sulfate expression by cytokines., Grant value (£): 1,600

Teaching qualification:

Title of qualification gained: Postgraduate Certificate in Higher Education