Liverpool Skylilne

Pharmacy and Biomolecular Sciences

Dr Femi Olorunniji

Dr Femi Olorunniji

Telephone: 0151 231 2116

ORCID

Biography

I am a Senior Lecturer in Biotechnology in the School of Pharmacy & Biomolecular Sciences, Liverpool John Moores University. My research interest is in protein engineering and their applications in Synthetic Biology. We aim to engineer novel protein functions for use as molecular tools/devices in metabolic pathway engineering, genome editing, genetic switches and memory devices.

Current research projects include:
1. Site-specific recombinases: Reaction mechanisms and applications in synthetic biology. With specific interest in serine integrases and their Recombinaton Directionality Factors (RDFs), we are exploring new ways of altering the target specificity of these recombinases as well as novel ways of controlling their activities for synthetic biology applications.
2. Development of novel transcriptional logic gates. We are adapting a natural transcription regulatory system associated with phage inducible pathogenicity islands (PICIs) to develop custom-built transcription factors that can serve as building blocks for genetic logic gates
3. Design and applications of catalytic DNA nanostructures. Using simple design approaches, we are investigating structural features that enhance the catalytic efficiencies of G-quadruplex-hemin complexes that display peroxidase activities. Our ultimate aim is to design better DNAzymes with biosensing applications.
4. Reaction mechanisms of bacterial PHP protein tyrosine phosphatases. We are employing domain swapping and other protein engineering strategies to understand the catalytic mechanisms and probe possible applications of this class of bacterial PTPs in biotechnology.

We welcome new PhD applications in these areas.

Academic appointments

Senior Lecturer in Biotechnology, Liverpool John Moores University, Liverpool John Moores University, 2017 - present

Publications

Highlighted publications

Olorunniji FJ, Lawson-Williams M, McPherson AL, Paget JE, Stark WM, Rosser SJ. Control of ϕC31 integrase-mediated site-specific recombination by protein trans-splicing Nucleic Acids Research, DOI Author Url Public Url

Journal article

Adeoye RI, Osalaye DS, Ralebitso-Senior TK, Boddis A, Reid AJ, Fatokun AA, Powell AK, Malomo SO, Olorunniji FJ. 2019. Catalytic Activities of Multimeric G-Quadruplex DNAzymes Catalysts, 9 :613-613 DOI Author Url Public Url

Olorunniji FJ, McPherson AL, Rosser SJ, Smith MCM, Colloms SD, Stark WM. 2017. Control of serine integrase recombination directionality by fusion with the directionality factor Nucleic Acids Research, 45 :8635-8645 DOI Author Url Publisher Url Public Url

Olorunniji FJ, Rosser SJ, Stark WM. 2016. Site-specific recombinases: molecular machines for the Genetic Revolution BIOCHEMICAL JOURNAL, 473 :673-684 DOI Author Url

Olorunniji FJ, McPherson AL, Pavlou HJ, McIlwraith MJ, Brazier JA, Cosstick R, Stark WM. 2015. Nicked-site substrates for a serine recombinase reveal enzyme-DNA communications and an essential tethering role of covalent enzyme-DNA linkages NUCLEIC ACIDS RESEARCH, 43 :6134-6143 DOI Author Url

Yakubu MT, Sunmonu TO, Lewu FB, Ashafa AOT, Olorunniji FJ, Eddouks A. 2015. Medicinal Plants Used in the Management of Diabetes Mellitus 2015 EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, DOI Author Url

Colloms SD, Merrick CA, Olorunniji FJ, Stark WM, Smith MCM, Osbourn A, Keasling JD, Rosser SJ. 2014. Rapid metabolic pathway assembly and modification using serine integrase site-specific recombination NUCLEIC ACIDS RESEARCH, 42 DOI Author Url

Yakubu MT, Sunmonu TO, Lewu FB, Ashafa AOT, Olorunniji FJ, Eddouks M. 2014. Efficacy and Safety of Medicinal Plants Used in the Management of Diabetes Mellitus EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, DOI Author Url

Olorunniji FJ, Buck DE, Colloms SD, McEwan AR, Smith MCM, Stark WM, Rosser SJ. 2012. Gated rotation mechanism of site-specific recombination by phi C31 integrase PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 109 :19661-19666 DOI Author Url

Igunnu A, Osalaye DS, Olorunsogo OO, Malomo SO, Olorunniji FJ. 2011. Distinct metal ion requirements for the phosphomonoesterase and phosphodiesterase activities of calf intestinal alkaline phosphatase Open Biochemistry Journal, 5 :67-72 DOI Author Url

Prorocic MM, Dong W, Olorunniji FJ, Akopian A, Schloetel J-G, Hannigan A, McPherson AL, Stark WM. 2011. Zinc-finger recombinase activities in vitro NUCLEIC ACIDS RESEARCH, 39 :9316-9328 DOI Author Url

Stark WM, Boocock MR, Olorunniji FJ, Rowland S-J. 2011. Intermediates in serine recombinase-mediated site-specific recombination BIOCHEMICAL SOCIETY TRANSACTIONS, 39 :617-622 DOI Author Url

Olorunniji FJ, Stark WM. 2010. Catalysis of site-specific recombination by Tn3 resolvase BIOCHEMICAL SOCIETY TRANSACTIONS, 38 :417-421 DOI Author Url

Olorunniji FJ, Stark WM. 2009. The catalytic residues of Tn3 resolvase NUCLEIC ACIDS RESEARCH, 37 :7590-7602 DOI Author Url

Olorunniji FJ, He J, Wenwieser SVCT, Boocock MR, Stark WM. 2008. Synapsis and catalysis by activated Tn3 resolvase mutants NUCLEIC ACIDS RESEARCH, 36 :7181-7191 DOI Author Url

Olorunniji FJ, Malomo SO, Adediran SA, Odutuga AA. 2000. Promethazine oxidation by redox mediation in peroxidase reactions ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 380 :251-256 DOI Author Url

Malomo SO, Olorunniji FJ. 1999. Inhibition of myeloperoxidase by acetylsalicylic acid: Alternative anti-inflammatory mechanism BIOMEDICAL LETTERS, 60 :13-17 Author Url

Olorunniji FJ, Lawson-Williams M, McPherson AL, Paget JE, Stark WM, Rosser SJ. Control of ϕC31 integrase-mediated site-specific recombination by protein trans-splicing Nucleic Acids Research, DOI Author Url Public Url

Chapters

Olorunniji FJ, Merrick C, Rosser SJ, Smith MCM, Stark WM, Colloms SD. 2017. Multipart DNA assembly using site-specific recombinases from the large serine integrase family Methods in Molecular Biology 1642 :303-323 DOI Author Url

Olorunniji FJ, Rosser SJ, Marshall Stark W. 2017. Purification and in vitro characterization of zinc finger recombinases Methods in Molecular Biology 1642 :229-245 DOI Author Url