Dr Craig Wilding
School of Biological and Environmental Sciences
Faculty of Science
Email: C.S.Wilding@ljmu.ac.uk
Telephone: 0151 231 2500
I have over twenty years of experience of the application of genetic and molecular biological techniques to the study of evolutionary biology, population genetics, human genetics and vector biology. I obtained a degree in Marine Biology from the University of Wales (Bangor) and subsequently completed my PhD research on the ‘Population genetics of the scallop Pecten maximus’. Following completion of my PhD I worked in evolutionary genetics at the University of Leeds before moving into Industry where I investigated human radiobiological genetics and genetic biomarkers of radiation exposure at Westlakes Research Institute. In 2006 I moved to the Liverpool School of Tropical Medicine (LSTM) to investigate the genetic mechanisms of insecticide resistance in mosquito vectors of human disease. After eight years at LSTM I joined LJMU as a lecturer in Evolutionary Genetics.
The main focus of my research is the genetic basis of insecticide resistance in mosquitoes. Resistance to the insecticides used in vector control represents a possible impediment to effective control strategies and an understanding of the genetic basis of this resistance would aid not only in the development of improved insecticide formulations, and hence more effective control measures, but also allow the development of genetic tests to rapidly measure the extent and spread of resistance. My work has used a variety of approaches including whole genome Agilent gene expression microarrays, qPCR, association mapping using the Illumina GoldenGate assay, targeted re-sequencing, and in vitro expression of identified candidates in bacterial and insect cell lines. I am particularly interested in methods to identify the regulatory variants underpinning the gene expression changes seen in insecticide resistant mosquitoes and to this end use reporter assays to study the activity of promoter sequences of candidate detoxification genes, and am involved in a project to characterise the microRNA repertoire (key post transcriptional regulators of genes) in An. gambiae (with Rodolphe Poupardin and Hilary Ranson, LSTM).
Recent work on insecticide resistance in An. gambiae and An. arabiensis has been conducted as part of the East African ICEMR (International Centre of Excellence for Malaria Research), based in Uganda and studying three key collection sites – Tororo, Jinja and Kanungu. I continue to have links with this programme. See http://www.niaid.nih.gov/labsandresources/resources/icemr/Pages/uganda.aspx
Additionally, I am involved, through the Donnelly group at LSTM, in a collaboration with the Kwiatkowski group at the Wellcome Trust Sanger Institute – the Anopheles 1000 genome initiative – undertaking whole genome sequencing of field-collected samples and lab colonies of Anopheles gambiae to study diversity and population structure of malaria vectors across a range of spatial scales in sub Saharan Africa. Through the Donnelly group I am also working on the annotation and analysis of insecticide resistance-associated gene families across a number of recently sequenced Anopheles mosquito species as part of the Anopheles genome cluster (see https://agcc.vectorbase.org/index.php/Main_Page).
I continue to have interests in the evolutionary genetics of reproductive isolation in the intertidal gastropod Littorina saxatilis. On some UK shores this snail exists as two morphotypes with a thin shelled morph (the H form) found high on the shore on cliffs and large boulders and a thick shelled morph (the M form) found low on the shore on and under boulders and on bedrock. Gene flow appears to be restricted, though possible, between these two forms. These snails are an interesting example of parapatric speciation and previous work utilising amplified fragment length polymorphism (AFLP) markers (Wilding et al. 2001) investigated the proportion of the genome responsible for this reproductive isolation. This was one of the first practical uses of outlier analysis to identify regions of the genome under selection. With the recent sequencing of the Littorina genome there is the potential to continue research on the genetic basis of this fascinating system.
Recently, I have also become interested in the genetics of reproductive isolation in two species of Tegenaria house spiders present throughout much of England and Wales. These species (Tegenaria saeva and T. gigantea) inhabit the west of England and Wales, and Eastern England respectively meeting in a hybrid zone which roughly follows the Welsh border. Additionally, I am interested in evaluating the extent of reproductive isolation between colour morphs of the beadlet anemone Actinia equina. To this end I am currently sequencing the genome of this species.
Degrees
1996, Bangor University, United Kingdom, Ph.D
1992, Bangor University, United Kingdom, BSc (Hons)
Academic appointments
Senior Postdoctoral Research Associate, Department of Vector Biology, Liverpool School of Tropical Medicine, 2006 - 2013
Post-doctoral researcher, Biological Science, University of Leeds, 1996 - 2002
Journal article
Hedtke SM, Post RJ, Feleke SM, Gebretsadik FS, Boakye DA, Krueger A, Grant WN, Wilding CS. 2024. Cytotaxonomic characterization and estimation of migration patterns of onchocerciasis vectors (Simulium damnosum sensu lato) in northwestern Ethiopia based on RADSeq data Cotton J. PLoS Neglected Tropical Diseases, 18 :e0011868 DOI Author Url Publisher Url Public Url
Howarth ERI, Szott ID, Witham CL, Wilding CS, Bethell EJ. 2023. Genetic polymorphisms in the serotonin, dopamine and opioid pathways influence social attention in rhesus macaques (Macaca mulatta) Singh AS. PLoS One, 18 :1-29 DOI Author Url Publisher Url Public Url
Trissi N, Troczka BJ, Ozsanlav-Harris L, Singh KS, Mallott M, Aishwarya V, O'Reilly A, Bass C, Wilding CS. 2023. Differential regulation of the Tor gene homolog drives the red/green pigmentation phenotype in the aphid Myzus persicae. Insect Biochemistry and Molecular Biology, 153 DOI Author Url Publisher Url Public Url
Vendrami DLJ, Hoffman JI, Wilding CS. 2022. Heterogeneous Genomic Divergence Landscape in Two Commercially Important European Scallop Species Genes, 14 DOI Author Url Publisher Url Public Url
Shaw HJ, Wilding CS. 2021. A new microsporidian parasite of the genus Amblyospora (Hazard and Oldacre, 1975) identified from the halophilic mosquito Ochlerotatus detritus (Haliday, 1833) (Diptera: Culicidae) through rDNA ITS sequencing Aquatic Insects, 42 :37-49 DOI Author Url Publisher Url Public Url
Xue AT, Schrider DR, Kern AD, Della Torre A, Caputo B, Kabula B, White B, Godfray C, Edi C, Wilding C, Neafsey D, Conway D, Weetman D, Ayala D, Kwiatkowski D, Sharakhov I, Midega J, Xu J, Pinto J, Essandoh J, Matowo J, Vernick K, Djogbenou LS, Coulibaly M, Lawniczak M, Donnelly M, Hahn M, Fontaine M, Riehle M, Besansky N, Cornejo O, Mccann R, O'loughlin S, Robert V, Xue A, Miles A, Clarkson C, Battey CJ, Champion C, Labbe F, Bottà G, Adrion J, Nelson J, Harding N, Wang R, Small ST, Redmond S, Antão T. 2020. Discovery of Ongoing Selective Sweeps within Anopheles Mosquito Populations Using Deep Learning Falush DANIEL. Molecular Biology and Evolution, 38 :1168-1183 DOI Author Url Publisher Url Public Url
The Anopheles gambiae 1000 Genomes Consortium . 2020. Genome variation and population structure among 1142 mosquitoes of the African malaria vector species Anopheles gambiae and Anopheles coluzzii Genome Research, 30 :1533-1546 DOI Author Url Publisher Url Public Url
Edmunds C, Wilding CS, Rae R. 2020. Pathogenicity and environmental tolerance of commercial and UK native entomopathogenic nematodes (Steinernema and Heterorhabditis spp.) to the larvae of mosquitoes (Aedes aegypti and Ochlerotatus detritus) International Journal of Pest Management, DOI Author Url Publisher Url Public Url
Wilding CS, Fletcher N, Smith EK, Prentis P, Weedall GD, Stewart Z. 2020. The genome of the sea anemone Actinia equina (L.): Meiotic toolkit genes and the question of sexual reproduction Marine Genomics, 53 DOI Author Url Publisher Url Public Url
Silva Martins WF, Wilding CS, Isaacs AT, Rippon EJ, Megy K, Donnelly MJ. 2019. Transcriptomic analysis of insecticide resistance in the lymphatic filariasis vector Culex quinquefasciatus Scientific Reports, 9 DOI Author Url Publisher Url Public Url
Silva Martins WF, Silva Pereira BN, Vieira Alves AT, Murphy A, Silva Martins PG, Weetman D, Wilding CS, Donnelly MJ. 2019. Development and application of a tri-allelic PCR assay for screening Vgsc-L1014F kdr mutations associated with pyrethroid and organochlorine resistance in the mosquito Culex quinquefasciatus Parasites & Vectors, 12 DOI Author Url Publisher Url Public Url
Wilding C. 2018. The genetic basis of size in pet dogs: The study of quantitative genetic variation in an undergraduate laboratory practical Biochemistry and Molecular Biology Education, 46 :623-629 DOI Author Url Publisher Url Public Url
Wilding CS, Weedall GD. 2018. Morphotypes of the common beadlet anemone Actinia equina (L.) are genetically distinct Journal of Experimental Marine Biology and Ecology, 510 :81-85 DOI Author Url Publisher Url Public Url
Brown FV, Logan RAE, Wilding CS. 2018. Carbamate resistance in a UK population of the halophilic mosquito Ochlerotatus detritus implicates selection by agricultural usage of insecticide International Journal of Pest Management, 65 :284-292 DOI Author Url Publisher Url Public Url
Edmunds C, Post RJ, Wilding CS, Rae R. 2018. A survey investigating the diversity and distribution of entomopathogenic nematodes in the UK and the first confirmed UK record of Steinernema carpocapsae Nematology, 20 :851-858 DOI Author Url Publisher Url Public Url
Wynne R, Wilding CS. 2018. Mitochondrial DNA haplotype diversity and origin of captive sand tiger sharks (Carcharias taurus) Journal of Zoo and Aquarium Research, 6 :74-78 DOI Publisher Url Public Url
Wilding CS. 2018. Regulating resistance: CncC:Maf, antioxidant response elements and the overexpression of detoxification genes in insecticide resistance Current Opinion in Insect Science, 27 :89-96 DOI Author Url Publisher Url Public Url
Weetman D, Wilding CS, Neafsey DE, Müller P, Ochomo E, Isaacs AT, Steen K, Rippon EJ, Morgan JC, Mawejje HD, Rigden DJ, Okedi LM, Donnelly MJ. 2018. Candidate-gene based GWAS identifies reproducible DNA markers for metabolic pyrethroid resistance from standing genetic variation in East African Anopheles gambiae. Scientific Reports, 8 DOI Author Url Publisher Url Public Url
Wilding CS. 2017. Genetic diversity of the African malaria vector Anopheles gambiae Nature, 552 :96-100 DOI Author Url Publisher Url Public Url
Edmunds C, Wilding CS, Rae R. 2017. Susceptibility of Chironomus plumosus larvae (Diptera: Chironomidae) to entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae): potential for control European Journal of Entomology, 114 :526-532 DOI Author Url Publisher Url Public Url
Martins WSM, Wilding CS, Steen K, Mawejje H, Antão TR, Donnelly MJ. 2017. Local selection in the presence of high levels of gene flow: Evidence of heterogeneous insecticide selection pressure across Ugandan Culex quinquefasciatus populations PLoS Neglected Tropical Diseases, 11 DOI Author Url Publisher Url Public Url
Martins WFS, Subramaniam K, Steen K, Mawejje H, Liloglou T, Donnelly MJ, Wilding CS. 2017. Detection and quantitation of copy number variation in the voltage-gated sodium channel gene of the mosquito Culex quinquefasciatus Scientific Reports, 7 :1-10 DOI Author Url Publisher Url Public Url
Gomes B, Wilding CS, Weetman D, Sousa CA, Novo MT, Savage HM, Almeida APG, Pinto J, Donnelly MJ. 2015. Limited genomic divergence between intraspecific forms of Culex pipiens under different ecological pressures BMC Evolutionary Biology, 15 :197 DOI Author Url Publisher Url Public Url
Weetman D, Mitchell SN, Wilding CS, Birks DP, Yawson AE, Essandoh J, Mawejje HD, Djogbenou LS, Steen K, Rippon EJ, Clarkson CS, Field SG, Rigden DJ, Donnelly MJ. 2015. Contemporary evolution of resistance at the major insecticide target site gene Ace-1 by mutation and copy number variation in the malaria mosquito Anopheles gambiae. Molecular Ecology, DOI Author Url Publisher Url Public Url
Wilding CS, Weetman D, Rippon EJ, Steen K, Mawejje HD, Barsukov I, Donnelly MJ. 2015. Parallel evolution or purifying selection, not introgression, explains similarity in the pyrethroid detoxification linked GSTE4 of Anopheles gambiae and An. arabiensis MOLECULAR GENETICS AND GENOMICS, 290 :201-215 DOI Author Url Publisher Url Public Url
Neafsey DE, Waterhouse RM, Abai MR, Aganezov SS, Alekseyev MA, Allen JE, Amon J, Arca B, Arensburger P, Artemov G, Assour LA, Basseri H, Berlin A, Birren BW, Blandin SA, Brockman AI, Burkot TR, Burt A, Chan CS, Chauve C, Chiu JC, Christensen M, Costantini C, Davidson VLM, Deligianni E, Dottorini T, Dritsou V, Gabriel SB, Guelbeogo WM, Hall AB, Han MV, Hlaing T, Hughes DST, Jenkins AM, Jiang X, Jungreis I, Kakani EG, Kamali M, Kemppainen P, Kennedy RC, Kirmitzoglou IK, Koekemoer LL, Laban N, Langridge N, Lawniczak MKN, Lirakis M, Lobo NF, Lowy E, MacCallum RM, Mao C, Maslen G, Mbogo C, McCarthy J, Michel K, Mitchell SN, Moore W, Murphy KA, Naumenko AN, Nolan T, Novoa EM, O'Loughlin S, Oringanje C, Oshaghi MA, Pakpour N, Papathanos PA, Peery AN, Povelones M, Prakash A, Price DP, Rajaraman A, Reimer LJ, Rinker DC, Rokas A, Russell TL, Sagnon N, Sharakhova MV, Shea T, Simao FA, Simard F, Slotman MA, Somboon P, Stegniy V, Struchiner CJ, Thomas GWC, Tojo M, Topalis P, Tubio JMC, Unger MF, Vontas J, Walton C, Wilding CS, Willis JH, Wu Y-C, Yan G, Zdobnov EM, Zhou X, Catteruccia F, Christophides GK, Collins FH, Cornman RS, Crisanti A, Donnelly MJ, Emrich SJ, Fontaine MC, Gelbart W, Hahn MW, Hansen IA, Howell PI, Kafatos FC, Kellis M, Lawson D, Louis C, Luckhart S, Muskavitch MAT, Ribeiro JM, Riehle MA, Sharakhov IV, Tu Z, Zwiebel LJ, Besansky NJ. 2015. Highly evolvable malaria vectors: The genomes of 16 Anopheles mosquitoes SCIENCE, 347 :1258522-125822 DOI Author Url Publisher Url Public Url
Weetman D, Steen K, Rippon EJ, Mawejje HD, Donnelly MJ, Wilding CS. 2014. Contemporary gene flow between wild An. gambiae s.s. and An. arabiensis PARASITES & VECTORS, 7 DOI Author Url Publisher Url Public Url
Abdalla H, Wilding CS, Nardini L, Pignatelli P, Koekemoer LL, Ranson H, Coetzee M. 2014. Insecticide resistance in Anopheles arabiensis in Sudan: temporal trends and underlying mechanisms Parasites and Vectors, 7 DOI Author Url Publisher Url Public Url
Chrismas NAM, Torres-Fabila B, Wilding CS, Grahame JW. 2014. An association of mitochondrial haplotype with shell shape in the intertidal gastropod Littorina saxatilis JOURNAL OF MOLLUSCAN STUDIES, 80 :184-189 DOI Author Url Publisher Url Public Url
Mitchell SN, Rigden DJ, Dowd AJ, Lu F, Wilding CS, Weetman D, Dadzie S, Jenkins AM, Regna K, Boko P, Djogbenou L, Muskavitch MAT, Ranson H, Paine MJI, Mayans O, Donnelly MJ. 2014. Metabolic and Target-Site Mechanisms Combine to Confer Strong DDT Resistance in Anopheles gambiae PLOS ONE, 9 DOI Author Url Publisher Url Public Url
Bass C, Zimmer CT, Riveron JM, Wilding CS, Wondji CS, Kaussmann M, Field LM, Williamson MS, Nauen R. 2013. Gene amplification and microsatellite polymorphism underlie a recent insect host shift Proceedings of the National Academy of Sciences, 110 :19460-19465 DOI Author Url Publisher Url
Mawejje HD, Wilding CS, Rippon EJ, Hughes A, Weetman D, Donnelly MJ. 2013. Insecticide resistance monitoring of field-collected anopheles gambiae s.l. populations from jinja, eastern uganda, identifies high levels of pyrethroid resistance Medical and Veterinary Entomology, 27 :276-283 DOI Author Url Publisher Url Public Url
Wilding CS, Smith I, Lynd A, Yawson AE, Weetman D, Paine MJI, Donnelly MJ. 2012. A cis-regulatory sequence driving metabolic insecticide resistance in mosquitoes: functional characterisation and signatures of selection Insect Biochemistry and Molecular Biology, 42 :699-707 DOI Author Url Publisher Url Public Url
Jones CM, Liyanapathirana M, Agossa FR, Weetman D, Ranson H, Donnelly MJ, Wilding CS. 2012. Footprints of positive selection associated with a mutation (N1575Y) in the voltage-gated sodium channel of Anopheles gambiae Proceedings of the National Academy of Sciences of the United States of America, 109 :6614-6619 DOI Author Url Publisher Url Public Url
Mitchell SN, Stevenson BJ, Müller P, Wilding CS, Egyir-Yawson A, Field SG, Hemingway J, Paine MJI, Ranson H, Donnelly MJ. 2012. Identification and validation of a gene causing cross-resistance between insecticide classes in Anopheles gambiae from Ghana Proceedings of the National Academy of Sciences of the United States of America, 109 :6147-6152 DOI Author Url Publisher Url
Weetman D, Wilding CS, Steen K, Pinto J, Donnelly MJ. 2012. Gene flow-dependent genomic divergence between Anopheles gambiae M and S forms Molecular Biology and Evolution, 29 :279-291 DOI Author Url Publisher Url
Abilio AP, Kleinschmidt I, Rehman AM, Cuamba N, Ramdeen V, Mthembu DS, Coetzer S, Maharaj R, Wilding CS, Steven A, Coleman M, Hemingway J. 2011. The emergence of insecticide resistance in central Mozambique and potential threat to the successful indoor residual spraying malaria control programme Coleman M. Malaria Journal, 10 DOI Author Url Publisher Url Public Url
Lima EP, Paiva MHS, De Arúajo AP, Da Silva EVG, Da Silva UM, De Oliveira LN, Santana AEG, Barbosa CN, De Paiva Neto CC, Goulart MO, Wilding CS, Ayres CFJ, De Melo Santos MAV. 2011. Insecticide resistance in Aedes aegypti populations from Ceará, Brazil Parasites and Vectors, 4 DOI Author Url Publisher Url Public Url
Ayres CFJ, Müller P, Dyer N, Wilding CS, Rigden DJ, Donnelly MJ. 2011. Comparative genomics of the anopheline glutathione S-transferase epsilon cluster. PLoS One, 6 :e29237 DOI Author Url Publisher Url
Weetman D, Wilding CS, Steen K, Morgan JC, Simard F, Donnelly MJ. 2010. Association mapping of insecticide resistance in wild Anopheles gambiae populations: Major variants identified in a low-linkage disequilbrium genome PLoS ONE, 5 DOI Author Url Publisher Url Public Url
Wilding CS, Weetman D, Steen K, Donnelly MJ. 2009. Accurate determination of DNA yield from individual mosquitoes for population genomic applications Insect Science, 16 :361-363 DOI Author Url Publisher Url
Wilding CS, Weetman D, Steen K, Donnelly MJ. 2009. High, clustered, nucleotide diversity in the genome of Anopheles gambiae revealed through pooled-template sequencing: Implications for high-throughput genotyping protocols BMC Genomics, 10 DOI Author Url Publisher Url Public Url
Donnelly MJ, Corbel V, Weetman D, Wilding CS, Williamson MS, Black IV WC. 2009. Does kdr genotype predict insecticide-resistance phenotype in mosquitoes? Trends in Parasitology, 25 :213-219 DOI Author Url Publisher Url
Mzilahowa T, Ball AJ, Bass C, Morgan JC, Nyoni B, Steen K, Donnelly MJ, Wilding CS. 2008. Reduced susceptibility to DDT in field populations of Anopheles quadriannulatus and Anopheles arabiensis in Malawi: Evidence for larval selection Medical and Veterinary Entomology, 22 :258-263 DOI Author Url Publisher Url
Wilding CS, Curwen GB, Tawn EJ, Sheng X, Winther JF, Chakraborty R, Boice JF. 2007. Influence of polymorphisms at loci encoding DNA repair proteins on cancer susceptibility and G(2) chromosomal radiosensitivity.(vol 48, pg 48, 2007) ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 48 :502-502 DOI Author Url Publisher Url
Bass C, Williamson MS, Wilding CS, Donnelly MJ, Field LM. 2007. Identification of the main malaria vectors in the Anopheles gambiae species complex using a TaqMan real-time PCR assay Malaria Journal, 6 DOI Author Url Publisher Url Public Url
Wilding CS, Curwen GB, Tawn EJ, Sheng X, Winther JF, Chakraborty R, Boice JD. 2007. Influence of polymorphisms at loci encoding DNA repair proteins on cancer susceptibility and G2 chromosomal radiosensitivity. Environ Mol Mutagen, 48 :48-57 DOI Author Url Publisher Url
Grahame JW, Wilding CS, Butlin RK. 2006. Adaptation to a steep environmental gradient and an associated barrier to gene exchange in Littorina saxatilis. Evolution, 60 :268-278 DOI Author Url Publisher Url Public Url
Wilding CS, Rees GS, Relton CL, Tawn EJ. 2006. Genotype profiles of loci encoding DNA repair enzymes in newborn and elderly populations: no evidence of association with longevity. Biogerontology, 7 :35-41 DOI Author Url Publisher Url
Wilding CS, Cadwell K, Tawn EJ, Relton CL, Taylor GA, Chinnery PF, Turnbull DM. 2006. Mitochondrial DNA mutations in individuals occupationally exposed to ionizing radiation. Radiat Res, 165 :202-207 DOI Author Url Publisher Url Public Url
Wilding CS, Trikic MZ, Hingston JL, Copplestone D, Janet Tawn E. 2006. Mitochondrial DNA mutation frequencies in experimentally irradiated compost worms, Eisenia fetida Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 603 :56-63 DOI Author Url Publisher Url
Grahame JW, Wilding CS, Butlin RK. 2006. ADAPTATION TO A STEEP ENVIRONMENTAL GRADIENT AND AN ASSOCIATED BARRIER TO GENE EXCHANGE IN LITTORINA SAXATILIS Evolution, 60 :268-268 DOI Publisher Url
De Ruyck K, Wilding CS, Van Eijkeren M, Morthier R, Tawn EJ, Thierens H. 2005. Microsatellite polymorphisms in DNA repair genes XRCC1, XRCC3 and XRCC5 in patients with gynecological tumors: Association with late clinical radiosensitivity and cancer incidence Radiation Research, 164 :237-244 DOI Author Url Publisher Url Public Url
Simpson RJ, Wilding CS, Grahame J. 2005. Intron analyses reveal multiple calmodulin copies in Littorina. J Mol Evol, 60 :505-512 DOI Author Url Publisher Url Public Url
Wilding CS, Relton CL, Rees GS, Tarone RE, Whitehouse CA, Tawn EJ. 2005. DNA repair gene polymorphisms in relation to chromosome aberration frequencies in retired radiation workers. Mutat Res, 570 :137-145 DOI Author Url Publisher Url
Wilding CS, Relton CL, Sutton MJ, Jonas PA, Lynch S-A, Tawn EJ, Burn J. 2004. Thymidylate synthase repeat polymorphisms and risk of neural tube defects in a population from the northern United Kingdom. Birth Defects Res A Clin Mol Teratol, 70 :483-485 DOI Author Url Publisher Url
Relton CL, Wilding CS, Laffling AJ, Jonas PA, Burgess T, Binks K, Tawn EJ, Burn J. 2004. Low erythrocyte folate status and polymorphic variation in folate-related genes are associated with risk of neural tube defect pregnancy. Mol Genet Metab, 81 :273-281 DOI Author Url Publisher Url
Relton CL, Wilding CS, Pearce MS, Laffling AJ, Jonas PA, Lynch SA, Tawn EJ, Burn J. 2004. Gene-qene interaction in folate-related genes and risk of neural tube defects in a UK population Journal of Medical Genetics, 41 :256-260 DOI Author Url Publisher Url
Relton CL, Wilding CS, Jonas PA, Lynch SA, Tawn EJ, Burn J. 2003. Genetic susceptibility to neural tube defect pregnancy varies with offspring phenotype. Clin Genet, 64 :424-428 DOI Author Url Publisher Url
Wilding CS, Grahame J, Mill PJ. 2002. A GTT microsatellite repeat motif and differentiation between morphological forms of Littorina saxatilis: Speciation in progress? Marine Ecology Progress Series, 227 :195-204 DOI Author Url Publisher Url Public Url
Wilding CS, Butlin RK, Grahame J. 2001. Differential gene exchange between parapatric morphs of Littorina saxatilis detected using AFLP markers Journal of Evolutionary Biology, 14 :611-619 DOI Author Url Publisher Url Public Url
Wilding CS, Grahame J, Mill PJ. 2001. Correlation of morphological diversity with molecular marker diversity in the rough periwinkle Littorina saxatilis (Olivi) Journal of Shellfish Research, 20 :501-508 Author Url Public Url
Mill PJ, Clarke AP, Smith DC, Grahame J, Wilding CS. 2001. Lagoonal littorinids: Shell shape and speciation Journal of Shellfish Research, 20 :469-475 Author Url Public Url
Backeljau T, Frias Martins AM, Gosling E, Grahame J, Mill P, Brito C, Clarke A, Medeiros R, Small M, Wilding CS, Wilson I, Winnepenninckx B, Clarke R, de Wolf H. 2001. Periwinkles (Gastropoda, Littorinidae) as a model for studying patterns and dynamics of marine biodiversity Bulletin de L'Institut Royal des Sciences Naturelles de Belgique, Biologie, 71 :43-65 Public Url
Wilding CS, Grahame J, Mill PJ. 2000. Mitochondrial DNA CoI haplotype variation in sibling species of rough periwinkles. Heredity (Edinb), 85 ( Pt 1) :62-74 DOI Author Url Publisher Url
Wilding CS, Grahame J, Mill PJ. 2000. Mitochondrial DNA CoI haplotype variation in sibling species of rough periwinkles. Heredity, 85
Wilding CS, Grahame J, Mill PJ. 2000. Nuclear DNA restriction site polymorphisms and the phylogeny and population structure of an intertidal snail species complex (Littorina). Hereditas, 133 :9-18 DOI Author Url Publisher Url Public Url
Wilding CS, Beaumont AR, Latchford JW. 1999. Are Pecten maximus and Pecten jacobaeus different species? Journal of the Marine Biological Association of the United Kingdom, 79 :949-952 DOI Author Url Publisher Url
Simpson RJ, Wilding CS, Grahame J. 1999. Simple protocol for extracting nuclear DNA from single embryos of a marine snail. Biotechniques, 26 :1050-1052 DOI Author Url Publisher Url Public Url
Wilding CS, Mill PJ, Grahame J. 1999. Partial sequence of the mitochondrial genome of Littorina saxatilis: relevance to gastropod phylogenetics. J Mol Evol, 48 :348-359 DOI Author Url Publisher Url
Wilding CS, Grahame J, Mill PJ. 1998. Rough periwinkle polymorphism on the east coast of Yorkshire: Comparison of RAPD-DNA data with morphotype Hydrobiologia, 378 :71-78 DOI Author Url Publisher Url
Wilding CS, Latchford JW, Beaumont AR. 1998. An investigation of possible stock structure in Pecten maximus (L.) Using multivariate morphometrics, allozyme electrophoresis and mitochondrial DNA polymerase chain reaction-restriction fragment length polymorphism Journal of Shellfish Research, 17 :131-139 Author Url Public Url
Wilding CS, Grahame J, Mill PJ. 1998. Rough periwinkle polymorphism on the east coast of Yorkshire: Comparison of RAPD-DNA data with morphotype Hydrobiologia, 378 :71-78 DOI Publisher Url
Wilding CS, Beaumont AR, Latchford JW. 1997. Mitochondrial DNA variation in the scallop Pecten maximus (L.) assessed by a PCR-RFLP method. Heredity (Edinb), 79 ( Pt 2) :178-189 DOI Author Url Publisher Url
Wilding CS, Beaumont AR, Latchford JW. 1997. Mitochondrial DNA variation in the scallop Pecten maximus (L) assessed by a PCR-RFLP method HEREDITY, 79 :178-189 DOI Author Url Publisher Url
Thesis/Dissertation
Howarth E. 2021. ATTENTION BIAS: A NEW TOOL FOR WELFARE ASSESSMENT IN CAPTIVE RHESUS MACAQUES Bethell E, Wilding C, Engelhardt A. Public Url
Conference publication
Howarth E, Engelhardt A, Wilding C, Bethell EJ. 2017. Attention Bias: A New Tool for Welfare Assessment in Captive Rhesus Primate Eye, Primate Society of Great Britain 50th Anniversary 123 :46-46
Kabula BI, Matowo J, Batengana B, Wilding CS, Rippon E, Steen K, Kisinza W, Magesa S, Mosha F, Donnelly MJ. 2017. EVIDENCE OF MULTIPLE DDT RESISTANCE MECHANISMS IN THE MALARIA VECTOR ANOPHELES GAMBIAE FROM DAR ES SALAAM, TANZANIA AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 66th Annual Meeting of the American-Society-of-Tropical-Medicine-and-Hygiene (ASTMH) 97 :57-57 Author Url
preprint
Miles A, Harding NJ, Botta G, Clarkson C, Antao T, Kozak K, Schrider D, Kern A, Redmond S, Sharakhov I, Pearson R, Bergey C, Fontaine M, Troco A, Diabate A, Costantini C, Rohatgi K, Elissa N, Coulibaly B, Dinis J, Midega J, Mbogo C, Mawejje H, Stalker J, Rockett K, Drury E, Mead D, Jeffreys A, Hubbart C, Rowlands K, Isaacs A, Jyothi D, Malangone C, Vauterin P, Jeffrey B, Wright I, Hart L, Kluczynski K, Cornelius V, MacInnis B, Henrichs C, Giacomantonio R, Ayala D, Bejon P, Besansky N, Burt A, Caputo B, della Torre A, Godfray C, Hahn M, Neafsey D, O Loughlin S, Pinto J, Riehle M, Vernick K, Weetman D, Wilding C, White B, Lawniczak M, Donnelly M, Kwiatkowski D. Natural diversity of the malaria vector Anopheles gambiae DOI Publisher Url
Dyer NA, Lucas ER, Nagi SC, McDermott DP, Brenas JH, Miles A, Clarkson CS, Mawejje HD, Wilding CS, Halfon MS, Asma H, Heinz E, Donnelly MJ. Mechanisms of transcriptional regulation in Anopheles gambiae revealed by allele specific expression. DOI Author Url Publisher Url
Conference presentation:
Dissecting a stable hybrid zone: RNASeq comparison of the sensory transcriptome in allopatric and sympatric populations of Eratigena saeva and Eratigena duellica, Spider genomics, development and evolution, Van Mildert College, Durham University, Oral presentation. 2023
External PGR examinations performed:
University of Manchester, PhD, Target site (knockdown resistance) gene mutations and population genetic structure of Aedes aegypti in Saudi Arabia. 2023
University of Exeter, PhD, The biological and molecular factors influencing control of Trialeurodes vaporariorum and Bemisia tabaci on different host plants. 2020
University of Liverpool, PhD, Behavioural and physiological insecticide resistance of dengue vectors in the kingdom of Saudi Arabia. 2019
University of Exeter, PhD, Understanding the molecular and biochemical basis of insecticide selectivity against solitary bee pollinators. 2018
University of Liverpool, PhD, A genomic approach to understanding metabolic insecticide resistance to diamides in diamondback moth (Plutella xylostella). 2017
Research Grants Awarded:
LinnéSys fund (Linnaen Society/Systematics Association), Molecular investigation of the Actinia equina species complex, Grant value (£): 995.60. 2022
Wellcome Trust, Wellcome Trust Biomedical Vacation Scholarship: "Microsporidian parasites of the halophilic mosquito Ochlerotatus detritus: population distribution and role in defence against entomopathogenic nematodes", Grant value (£): 2768, Duration of research project: 8 weeks. 2019
GCRF (Global Challenges Research Fund), Examining the extent of migration of vectors of onchocerciasis within and between transmission zones in Ethiopia using RAD-Seq markers, Dr Rory Post, Grant value (£): £13,782, Duration of research project: 6 months. 2018
Global Challenge Research Fund, Examining the extent of migration of vectors of onchocerciasis within and between transmission zones in Ethiopia using RAD-Seq markers, Dr Rory Post, Grant value (£): 13780. 2018
Faculty of Science Technology Directorate Voucher Scheme, Genome sequencing of the sea anemone Actinia equina, a platform for evolutionary, developmental, and epigenetic studies, Dr Gareth Weedall, Grant value (£): 9682. 2018
Wellcome Trust, Wellcome Trust Biomedical Vacation Scholarship: "Cloning and sequencing of a carboxylesterase implicated in insecticide resistance in the malaria vector Anopheles arabiensis", Mr Daniel Kaptijn (LJMU Undergraduate), Grant value (£): 2000, Duration of research project: 8 weeks. 2015
Wellcome Trust, Wellcome Trust Biomedical Vacation Scholarship: "Development of novel molecular markers for the study of population structure in sub-Saharan African populations of Simulium blackflies, vectors of onchocerciasis", Ms. Calista Dufton-Kelly, LJMU undergraduate, Grant value (£): 2000, Duration of research project: 8 weeks. 2014
Other invited event:
Genetics and Genomics for the Third Generation, 3G Conference, Catrin Finch Centre, Wrexham, Invited presentation: Using mosquito genomes to aid malaria control efforts. 2018
Wales Gene Park Schools’ Genetics Roadshow, Coleg Menai, Ffriddoedd Road, Bangor, Gwynedd, Invited talk "The genomics of insecticide resistance in mosquitoes". 2017
Genetics for Teachers: CPD event, The Racecourse, Wrexham, Invited talk: "Unravelling species barriers – classroom applications of third generation DNA sequencing". 2017
Editorial boards:
Parasites & Vectors, Associate Editor, https://parasitesandvectors.biomedcentral.com/about/editorial-board. 2018
External PGR Supervision - completed students:
University of Liverpool, PhD, Evolutionary genetics of insecticide resistance in Culex quinquefasciatus. 2016
Teaching qualification:
Fellow of the Higher Education Academy. 2016
Staff and Educational Development Association: Professional Certificate in Supporting Learning. 2013